Why choose a CHO system instead of a HEK293 system?

Based on our expertise: The XtenCHO system is generally better suited for antibody production, with higher transient production yields observed compared to HEK293. The HEK293 system is often more appropriate for intracellular protein production. Additionally, it’s important to note that the majority of FDA-approved antibody-based drugs are produced using CHO expression systems, highlighting their reliability and effectiveness for antibody-related applications.

Do you have references of how XtenCHO™ was used ?

Here are a few publications and a patent that showcase how some of our customers have utilized XtenCHO : https://patents.google.com/patent/WO2024194298A1/en https://doi.org/10.3389/fimmu.2022.848571 https://doi.org/10.4049/jimmunol.2100595 https://doi.org/10.1186/s13195-022-01141-1 https://doi.org/10.1038/s41467-022-35700-9

What are the typical culture volumes ?

XtenCHO™ Race cells perform exceptionally well across various culture scales and are particularly efficient in low-volume productions. They can be used in formats such as 24-well deep-well plates, 50 mL vented flasks, 3 L flasks, and even in bioreactors exceeding 10 L. We provide an optimized protocol with recommended volumes tailored to different culture sizes to ensure optimal performance.

What is the composition of the Expression Medium?

The XtenCHO™ Expression Medium is serum-free, animal-component-free, and chemically defined. It requires additional supplementation with L-Glutamine and an anti-clumping agent both before and after transfection to ensure optimal performance.

What do we need to reorder after purchasing the starter kit?

After purchasing the starter kit, you’ll need to reorder consumables such as the XtenCHO™ Expression Medium, L-Glutamine, and anti-clumping agent as needed to continue your experiments. The Starter Kit provides enough materials for 1L of production. Once used, you can reorder the following components for additional 1L productions: XtenCHO™ Expression Medium (PX-XTE-002) XtenCHO™ Transfection Kit (PX-XTE-R-003)

Do you have stocks of reagents available?

Yes, we do. We maintain a stock of all the components for our kits, ensuring worldwide delivery within 2 to 3 days.

What is the expiration date for each item?

Each item is stable for 12 months from the date of manufacture if the cells, reagents, and medium are stored at their optimal storage temperatures. Diluted XtenFect Reagent, the working solution, and XtenCHO™ Race Enhancer should be used within 4 weeks after dilution or thawing.

Can we use our own plasmids ?

We recommend using the pXten1 Expression Vector for expressing your protein of interest. While XtenCHO™ Race Cells support high expression levels from various expression plasmids, they perform best when pXten1 is used as the expression vector. The chosen vector must include the CMV promoter.

Can XtenCHO™ Race Cells be used to generate stable cell lines?

Our XtenCHO™ Race Cells are derived from CHO-K1 cells, making them compatible with standard CHO-K1 selection methods. However, please note that XtenCHO™ Race Cells are engineered to be resistant to geneticin. We recommend using an alternative selection method to establish stable cell lines.

Can the XtenCHO™ Expression Medium be used with CHO cell lines other than the XtenCHO™ Race Cell line?

Yes, it is possible to adapt your CHO cells to the XtenCHO™ Expression Medium. This adaptation may enhance the productivity of your CHO cells and support high-density growth. However, there is no guarantee that other CHO cell lines will achieve the same expression levels as the XtenCHO™ Race Cells. XtenCHO™ Race Cells have been specifically engineered for optimal protein expression. When combined with the pXten1 Expression Vector, the optimized protocol, and reagents, these cells enable extended transient gene expression by enhancing plasmid-driven expression and improving plasmid maintenance.

What is the delivery timeframe?

The XtenCHO™ Race Starter Kit arrives precisely when you need it, keeping your projects on schedule and ensuring smooth, uninterrupted productivity. Good to know : Free shipping in EU for orders>500€ Europe: 2-4 business days USA & Canada: 2-4 business days Rest of the world: 5-10 business days

Search results for "XtenCHO"

25 Products
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XtenCHO ™ Race Cell Bank Pack
- PX-XTE-R-005
Brand:

ProteoGenix

Size:

XtenCHO ™ Cell Bank Pack
$3,230.00
+ 3230 loyalty points

In Stock Add to cart
XtenCHO ™ Race Transfection kit
- PX-XTE-R-003
Brand:

ProteoGenix

Size:

XtenCHO ™ Transfection kit
$902.00
+ 902 loyalty points

In Stock Add to cart
XtenCHO ™ Race Cells
- PX-XTE-R-004
Brand:

ProteoGenix

Size:

XtenCHO ™ Cells
$1,880.00
+ 1880 loyalty points

In Stock Add to cart
XtenCHO ™ Race Starter kit
- PX-XTE-R-001
Brand:

ProteoGenix

Size:

XtenCHO ™ Starter kit
$2,321.00
+ 2321 loyalty points

In Stock Add to cart
XtenCHO ™ Expression medium
- PX-XTE-002
- 6 publications
Brand:

ProteoGenix

Size:

XtenCHO ™ Expression medium
$351.00
+ 351 loyalty points

In Stock Add to cart
XtenCHO ™ Expression medium pack (10 x 1L)
- PX-XTE-006
- 5 publications
Brand:

ProteoGenix

Size:

XtenCHO ™ Expression medium pack
$2,440.00
+ 2440 loyalty points

In Stock Add to cart
Anti-RBD-5 antibody (Imdevimab)
- PTXCOV-A553
- 1 review
- 2 publications
- Validated ELISA
Brand:

ProteoGenix

Size:

100ug, 1MG, 50ug
Original price was: $212.00.Current price is: $155.00.
+ 155 loyalty points

In Stock Select options This product has multiple variants. The options may be chosen on the product page
Anti-CoV-S2 domain (D8) antibody
- PTXCOV-A541A
Brand:

ProteoGenix

Size:

100ug, 1MG, 50ug
Original price was: $327.00.Current price is: $250.00.
+ 250 loyalty points

In Stock Select options This product has multiple variants. The options may be chosen on the product page
Anti-CoV-S2 domain (G5) antibody
- PTXCOV-A541B
Brand:

Proteogenix

Size:

100ug, 1MG, 50ug
Original price was: $345.00.Current price is: $250.00.
+ 250 loyalty points

In Stock Select options This product has multiple variants. The options may be chosen on the product page
Anti-CoV-S2 domain (D7) antibody
- PTXCOV-A541C
Brand:

Proteogenix

Size:

100ug, 1MG, 50ug
Original price was: $315.00.Current price is: $250.00.
+ 250 loyalty points

In Stock Select options This product has multiple variants. The options may be chosen on the product page