Recombinant Human ACBD5 Protein, N-His

Description of Recombinant Human ACBD5 Protein, N-His

Introduction to Recombinant Human ACBD5 Protein

Recombinant Human ACBD5 Protein, also known as acyl-CoA binding domain containing 5, is a highly conserved protein that plays a crucial role in lipid metabolism and transport. It is encoded by the ACBD5 gene and is expressed in various tissues, including the liver, brain, and adipose tissue. This protein has been extensively studied and has shown promising potential in various applications, making it a valuable tool in the field of biotechnology and medicine.

Structure of Recombinant Human ACBD5 Protein

The recombinant form of ACBD5 protein is produced through genetic engineering techniques, where the ACBD5 gene is inserted into a suitable expression vector and then expressed in a host cell. The resulting protein is a 26-kDa polypeptide consisting of 233 amino acids. It contains an N-terminal acyl-CoA binding domain (ACB domain) and a C-terminal transmembrane domain. The ACB domain is responsible for binding to various acyl-CoA esters, while the transmembrane domain anchors the protein to the endoplasmic reticulum (ER) membrane.

The crystal structure of recombinant human ACBD5 protein has been determined, revealing a highly conserved three-dimensional structure. The ACB domain consists of a central β-sheet surrounded by α-helices, forming a barrel-like structure. This unique structure allows the protein to interact with different types of acyl-CoA esters, making it a versatile binding protein. The transmembrane domain, on the other hand, forms a helical hairpin structure that spans the ER membrane.

Activity of Recombinant Human ACBD5 Protein

The main function of recombinant human ACBD5 protein is to bind to acyl-CoA esters and facilitate their transport to various cellular compartments. It has been shown to interact with a wide range of acyl-CoA esters, including long-chain fatty acids, cholesterol esters, and bile acids. This binding activity is essential for the proper regulation of lipid metabolism and transport in the cell.

In addition to its role in lipid transport, recombinant human ACBD5 protein has also been found to play a crucial role in autophagy, a process that helps in the removal of damaged or unnecessary cellular components. It has been shown to interact with the autophagy-related protein ATG16L1, facilitating the formation of autophagosomes and promoting autophagy.

Applications of Recombinant Human ACBD5 Protein

Recombinant human ACBD5 protein has a wide range of applications in biotechnology and medicine. One of its primary uses is in the study of lipid metabolism and transport. The ability of this protein to bind to different types of acyl-CoA esters makes it a valuable tool in understanding the role of lipids in various cellular processes and diseases.

Moreover, recombinant human ACBD5 protein has shown potential as a diagnostic and therapeutic target for various diseases. Studies have shown that mutations in the ACBD5 gene can lead to abnormal lipid metabolism, which is associated with diseases such as non-alcoholic fatty liver disease and atherosclerosis. Therefore, targeting this protein could potentially help in the development of new treatments for these diseases.

Furthermore, recombinant human ACBD5 protein has also been investigated for its potential in drug delivery. Due to its ability to interact with different types of acyl-CoA esters, it can be used as a carrier for delivering drugs to specific cellular compartments, such as the ER. This targeted drug delivery system has the potential to improve the efficacy and reduce the side effects of various drugs.

Conclusion

In conclusion, recombinant human ACBD5 protein is a versatile and essential protein in lipid metabolism and transport. Its unique structure and binding activity make it a valuable tool in various applications, including the study