Nycodenz AG®

Datasheet of Nycodenz AG®

Nycodenz AG® is a cell separation media that has been widely used in the field of biotechnology and life sciences. It is a non-ionic, low osmotic, and iodinated density gradient medium that is commonly used for various applications such as cell separation, organelle isolation, and virus purification.

Structure of Nycodenz AG®

Nycodenz AG® is a synthetic polymer that is made up of the compound N,N’-bis(acryloyl)cystamine. It has a molecular weight of 1,500 Da and a density of 1.32 g/ml. The polymer is hydrophilic in nature, with a hydroxyl group (-OH) at one end and an acryloyl group (-CH=CH2) at the other end. This unique structure allows Nycodenz AG® to form a stable gradient when mixed with a sample containing cells or particles of different densities.

Activity of this cell separation medium

The main activity of Nycodenz AG® lies in its ability to form a continuous density gradient. When a sample is added on top of a Nycodenz AG® gradient, the particles or cells will migrate through the gradient until they reach their respective equilibrium position. This is due to the difference in density between the sample and the gradient, which creates a buoyancy force that drives the particles or cells to their specific position.

Nycodenz AG® is also known for its low osmotic properties, making it suitable for use in biological systems. This means that it does not cause significant changes in the osmotic pressure of the sample, which can be harmful to cells and other biological components. In addition, Nycodenz AG® is also non-toxic and non-pyrogenic, making it safe for use in various biological applications.

Application of Nycodenz AG®

1. Cell Separation

One of the primary applications of Nycodenz AG® is in cell separation. It is commonly used to isolate specific cell types from a heterogeneous population, such as blood cells or cultured cells. The density gradient formed by Nycodenz AG® allows for the separation of cells based on their density, with the heavier cells settling at the bottom of the gradient and the lighter cells floating at the top. This technique is widely used in research and clinical settings for the isolation of pure cell populations.

2. Organelle Isolation

Nycodenz AG® is also commonly used for the isolation of organelles from cells. The density gradient formed by Nycodenz AG® allows for the separation of organelles based on their density, with the heavier organelles settling at the bottom of the gradient and the lighter organelles floating at the top. This technique is widely used in research and diagnostic settings for the isolation of pure organelle fractions.

3. Virus Purification

Nycodenz AG® has also been used for the purification of viruses. The density gradient formed by Nycodenz AG® allows for the separation of viruses from other cellular components, such as proteins and nucleic acids. This technique is widely used in research and diagnostic settings for the isolation of pure virus particles.

Conclusion

In conclusion, Nycodenz AG® is a versatile cell separation media that has found widespread use in various applications in the field of biotechnology and life sciences. Its unique structure, activity, and low osmotic properties make it an ideal choice for the isolation and purification of cells, organelles, and viruses. With its proven track record and safe properties, Nycodenz AG® continues to be a popular choice for cell separation and other related applications.