Should be stored between 4°C - 24°C in a sterile and dark environment. It can be stored up to 3 years. Avoid long exposures to sunlight ( NB. The effect of sunlight exposure is negligible when working with OptiPrep™ on a day to day basis). Before use invert the bottles several times to mix the contents.
Brand
Axis-Shield/Serumwerk
Composition
Iodixanol: 60% (w/v) in water
Advantages
Advantages of OptiPrep™ vs Other gradients
OptiPrep™ vs Histopaque & Percoll Percoll and Histopaque include one or several washing step(s) prior to their use. The removal step(s) is/are required to avoid the formation of density gradients which can be toxic to mammalian cells. Unlike Histopaque and Percoll, OptiPrep™ has no potential for bioaccumulation, therefore is non-toxic for the cell.OptiPrep™ has a better yield, purity and viability (92%, 86%, 54%) compared to Histopaque (58%, 61%, 54%).
OptiPrep™ vs CsCl (Caesium chloride) Particle virus number ratios are 100 times higher with OptiPrep™ compared to those recorded from CsCl gradients.
Description of OptiPrep™ Density Gradient Medium (Iodixanol)
About OptiPrep™
OptiPrep™ is a high-purity density gradient medium based on iodixanol, widely used for the separation and purification of biological particles using density gradient centrifugation. This ready-to-use OptiPrep density gradient solution enables efficient isolation of cells, viruses, organelles, and extracellular vesicles while maintaining physiological osmotic conditions.
The OptiPrep iodixanol density gradient medium provides excellent resolution and reproducibility during ultracentrifugation. Unlike traditional gradient materials, iodixanol solutions remain iso-osmotic across a wide range of densities, helping preserve biological activity and cellular integrity during separation procedures.
OptiPrep density gradients are commonly used in research laboratories for virus purification, exosome isolation, membrane fractionation, and organelle separation. The medium allows researchers to create either continuous or discontinuous density gradients, offering flexibility for many experimental protocols.
Manufactured under strict quality standards, OptiPrep™ ensures high purity, low endotoxin levels, and consistent performance for demanding biological applications. The solution is sterile filtered and designed for reliable use in density gradient centrifugation workflows.
Key Features
High-purity OptiPrep iodixanol density gradient medium
Iso-osmotic solutions across a broad density range
Ideal for ultracentrifugation and density gradient separations
Excellent resolution for virus, vesicle, and organelle purification
Sterile filtered and produced under controlled conditions
Typical Applications of OptiPrep Density Gradients
The OptiPrep density gradient medium is widely used in many biological and biomedical applications, including:
Thanks to its iodixanol formulation, OptiPrep density gradients provide improved viability and structural preservation compared to traditional sucrose or cesium chloride gradients.
Why Use OptiPrep Iodixanol Gradients?
OptiPrep iodixanol solutions offer several advantages over conventional density gradient media. Because iodixanol forms iso-osmotic gradients, biological samples experience minimal osmotic stress during centrifugation. This property is particularly important when isolating delicate structures such as viruses, extracellular vesicles, or subcellular organelles.
The OptiPrep density gradient medium also allows precise control over density ranges, enabling researchers to optimize separation conditions for their specific samples.
OptiPrep Density Gradient for Advanced Research
OptiPrep™ has become a standard reagent for density gradient ultracentrifugation in virology, cell biology, and molecular biology laboratories. Its versatility and reproducibility make it suitable for both routine laboratory workflows and advanced research protocols.
By providing a reliable OptiPrep density gradient solution, researchers can achieve high-resolution separations while maintaining sample integrity.
ProteoGenix ships OptiPrep™ quickly across the United States from our US warehouse, ensuring reliable supply and fast delivery for research laboratories.
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