With more than 15 years of extensive experience in custom protein expression, and over 1500 proteins successfully produced in 5 systems, ProteoGenix is a leader in difficult-to-express recombinant proteins and antibodies productions. READ MORE
We provide small to large-scale protein expression and purification services in each of or 5 platforms at competitive prices but also more advanced and customized packages in which we test for instance custom recombinant protein expression conditions in several systems in parallel or with extended protein production conditions in a given system. Our services include all the steps from gene synthesis and expression vector construction to pilot protein production in flasks and large-scale protein expression in fermenters and bioreactors.
Our High throughput protein expression condition screening is intended to overcome challenges related to solubility, purity, functionality, productivity, refolding or formulation. Such a commitment involves having access to, and mastering, all existing technologies, processes and tools available. With extensive experience in the expression of recombinant proteins of any kind, such as antibodies, enzymes, membrane proteins, cytokines, etc., and of any species, ProteoGenix has developed a complete toolbox to tackle any such challenge and to provide best-in-class protein engineering services. Try guaranteed packages from the most reliable protein production company of its kind.
EXPRESSION IN E. COLI
Periplasmic expression, Refolding tests, guaranteed
packages, endotoxin removal, Custom E. coli expression,
protein production in bacteria
IN MAMMALIAN CELLS
Transient expression, Recombinant antibody
production, SEC and IEX purifications,
Low endotoxin levels, CHO, HEK293, BHK
Low cost production,
HTP antibody variants
Stable cell line
STABLE CELL LINE
IP free systems, guaranteed yield, process
development, HEK293 and CHO stable
HIGH THROUGHPUT PROTEIN
For difficult-to-express proteins, 1000+
expression conditions, small-scale, high
Sf9, Sf21, Hi5, glycosylated proteins,
protein purification, baculovirus
PRODUCTION IN B. SUBTILIS
Low endotoxin level,
Recombinant protein secretion,
For unknown optimal system, tests in up
to 5 systems, purified protein delivery for
FERMENTATION / BIOREACTOR
LARGE – SCALE PROTEIN PRODUCTION
1 to 4000L in each 5 systems,
Large scale purification services,
Process development services
Custom Protein Production
” I recently chose ProteoGenix to produce a recombinant protein in HEK293 cells. The high productivity and strong activity of the protein obtained, probably due to proper glycosylation and folding, allowed us to generate exciting results in functional studies and reach new milestones in our research project. The quality of the service provided, and the smooth and kind communication I had with the account manager in charge of our project, were paramount to this success. I’m happy to recommend ProteoGenix for recombinant protein productions. “
Why Choose ProteoGenix for your Protein Expression?
Why Choose ProteoGenix
for your Protein Expression ?
More than 14 years of outstanding track record in the production of difficult-to-express proteins
5 available protein expression systems and complete toolbox to overcome any particular protein expression challenges
One-stop solution from gene to protein, process development and large scale production. Consequences: faster service, increased success rate, less administrative work.
IP-free strains and cell lines
Very small to large scale productions with 1 to 4000 L batch
Productivity of up to 800mg/L in transient expression and 5g/L in stable expression.
Claudiximab protein expression
A customer requested the recombinant expression of Claudiximab. He ordered a protein expression package including:
GENE SYNTHESIS AND SUBCLONING
The genes coding for the Heavy chain (HC) and the light chain (LC) of the therapeutic antibody Claudiximab were chemically synthesized with optimization for protein expression in mammalian cells. The genes were cloned in ProteoGenix’s proprietary mammalian cells expression vector pTXs1.
Figure 1. Purification profile of Claudiximab. Reduced PAGE with Coomassie blue staining.
IN. Input sample. FT. Flow through. W. Wash step. E1-E11. Eluted fractions.
Figure 2. Final QC of purified Claudiximab. Coomassie blue staining.
A. Non-reduced PAGE. B. Reduced PAGE
PROTEIN EXPRESSION RESULT
Which System choose for your Protein Production?
COMPARISON BETWEEN 5 EXPRESSION SYSTEMS PROVIDED BY PROTEOGENIX -
PROKARYOTIC VS EUKARYOTIC CELLS EXPRESSION SYSTEMS.
From a general standpoint, the table below gives an idea of the advantages of each five different heterologous protein expression systems for production of recombinant proteins. Of course, features can vary from one protein to another. The more arrows there are, the better it is.
|Time||Cost||PTM||Low Endotoxin levels||Simple process scale up||Secretion|
|Yeast (P. pastoris, |
The table below gives an idea of the advantages of each five different heterologous protein expression systems for production of recombinant proteins from a general standpoint. Of course features can vary from one protein to another. The more arrows there are, the better it is.
|Time||Cost||PTM||Low Endotoxin levels|
|Yeast (P. pastoris, |
|Simple process scale up||Secretion|
|Yeast (P. pastoris, |
TIME DURATION AND PRICE
|Expression system||Time duration||Price|
|E. coli||3-6 weeks||Starting from |
|B. subtilis||4-6 weeks|
|Yeast (P. pastoris, |
|Mammalian cells||7-9 weeks|
Small scale expression and purification evaluation with many expression conditions tested.
1L production and purification
NON EXHAUSTIVE MULTI-EXPRESSION SYSTEM PACKAGES
|ATX-PTR4S01||4 systems |
|E. coli / Yeast / Baculovirus / |
|Starting from |
|ATX-PTR3S05||3 system |
|B. subtilis/ Yeast / Baculovirus|
|ATX-PTR2S02||2 systems |
|E. coli / Baculovirus|
The most commonly used system of recombinant protein purification is E. coli. Indeed, it is the simplest, fastest and most cost effective system for custom expression of a protein. Bacterial cultures grow much faster than insect or mammalian cells or yeast. It is therefore easier and quicker to test a large number of different conditions. More solutions are also available for testing and therefore allow a higher success rate in expressing recombinant proteins. Transient expression yields of recombinant protein are also generally much higher than in yeast, baculovirus / insect cells or mammalian cells. Production of recombinant proteins in E. coli (Escherichia Coli) is often the preferred solution proposed by ProteoGenix when the final application requires large quantities of proteins such as crystallography and nuclear magnetic resonance (NMR) and no Post-Translational Modifications (PTM) are required. However, the main drawbacks of E. coli system are the high amounts of endotoxins remaining in the final sample and the lack of Post-Translational modifications. It is possible to remove endotoxins but it isn’t convenient at an industrial stage when there is not much to be done to solve PTM problems apart from using a different expression system. With these five systems, we are able to meet the requirements of a large number protein synthesis orders and we therefore have a success rate of over 95% in recombinant protein synthesis. We are constantly working on integrating new systems and solutions to provide the best level of service for anyone who needs to buy high quality recombinant protein production services.
Team, Capacity, Organization
If you have any doubts about which system to choose from, we, as a leading protein production manufacturer, can guide you from gene to protein and help you define an optimal strategy while taking into account your time constraints, budget and required protein features..
Our recombinant protein expression laboratories are organized across several platforms and supporting labs to ensure the best quality of service, the highest product purity, the lowest risk of contamination and the best organization for a fast and reliable service.
We have a platform for each of our five systems and a separate quality control lab, molecular biology lab, packing lab… Each platform is managed by experienced team leaders that ensure they deliver proteins corresponding to initial customer specifications. With more than 1500 proteins produced to date, and specialized teams for each system, our expertise is unrivalled.
Custom recombinant protein
ProteoGenix recombinant proteins are used in a wide spectrum of applications and our customers are usually involved in Life sciences, including Pharma companies, Diagnostics, Biotech, the Agri-Food Industry, Crop science, New energies, Cosmetics, National Research Laboratories, etc..
Below is a non-exhaustive list of applications: