XtenCHO ™ Race Transfection kit

Description of XtenCHO ™ Race Transfection kit

XtenCHO™ Race Transfection: Optimized for High-Yield Protein Production

Transfection is a fundamental step in recombinant protein production, enabling eukaryotic cells to express target proteins by incorporating nucleic acid sequences. While this process is essential, it can be costly and may impact cell viability. To overcome these challenges, optimized technologies are required to enhance efficiency and reproducibility.

ProteoGenix has developed XtenCHO™ Race, a proprietary CHO-K1-derived cell line tailored for high-yield recombinant protein production. To maximize the potential of this cell line, the XtenCHO™ Race Transfection Kit has been specifically designed to achieve exceptional transfection efficiency, ensuring researchers obtain maximum protein yields with a simplified workflow.

What’s Inside the XtenCHO™ Race Transfection Kit?

The XtenCHO™ Race Transfection Kit includes two key components:

-XtenFect Reagent: A transfection reagent optimized for XtenCHO™ Race cells grown in XtenCHO™ Expression Medium. It enables efficient nucleic acid uptake, maximizing recombinant protein production.
-XtenCHO™ Race Enhancer: A proprietary supplement that enhances cell viability and productivity in densely transfected cultures, prolonging protein production and increasing overall yield per liter of culture.

The XtenFect Reagent is provided as a 10x concentrated stock solution, which must be diluted using the included XtenFect Working Solution. Once prepared, it can be directly added to XtenCHO™ Race cell cultures, eliminating the need for pre-mixing with DNA, a common step required by conventional transfection reagents. This streamlined approach reduces handling time and enhances reproducibility.

Designed for 1 liter of culture, the XtenCHO™ Race Transfection Kit is animal-free and composed of chemically defined ingredients, ensuring consistency and regulatory compliance.

For a complete, ready-to-use solution, the XtenCHO™ Race Transfection Kit is also included in the XtenCHO™ Race Starter Kit, providing researchers with all the essential components to achieve high-yield recombinant protein expression efficiently and reliably.

XtenCHO™ Race outperforms up to 6X (Cixutumumab and Peresolimab)

Crude production yields in mg/L obtained in 24-well plates cultures without feeding (Octet Analysis) Yields in flask (better aeration) will be 1.5 to 2.5 Higher for Human/humanized IgG1/IgG4 (depending on the antibody considered)