Increase your antibody affinity by up to 1000 fold with ProteoGenix!
Increase your antibody affinity by up to 1000 fold!
Your affinity maturation project will benefit from 28 years of expertise in therapeutic antibody development in leading pharmaceutical companies.
Our priority is to ensure a safe investment to our customers! Our affinity maturation service includes unique guarantees determined after a in-depth preliminary study!
You get the full rights to the affinity matured antibodies at the end of the project!
To ensure efficient communication for your affinity maturation projects, you have highly skilled PhD account managers at your disposal.
Our phage display library design uses 3D modeling in order to limit irrelevant mutations and more specifically to keep the most appropriate mutations and avoid the generation of junk into the library.
Put your trust in a unique partner to help bring your therapeutic antibody project straight to the clinic!
ProteoGenix has developed a high-class affinity maturation service tailored to increase the affinity of your antibody against its target. Besides affinity, cross-reactivity can also be introduced to avoid the use of surrogate antibodies for preclinical development. Many other parameters can be measured and optimized such as:
To do so, site-directed mutagenesis or random mutagenesis is performed on the CDR regions.
Site-directed mutagenesis is based on 3D modeling in order to identify key residues involved in the antibody-antigen interaction. Specific mutations on these residues are performed in order to create a large phage display library with low junk variants and high probability of strong affinity enhancement.
Random mutagenesis are inserted by the use of error prone PCR. This method allows the construction of large libraries containing 108 starting from 3 to 10 selected antibodies.
As affinity maturation is fairly complex, our experts define the most relevant strategy after a preliminary study. This is the best way to guarantee our customers a safe investment and to include unique result guarantees to your project. This demonstrates our commitment to the success of your antibody affinity maturation.
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Affinity maturation is important in diagnostics and biotherapeutics as well as many other applications requiring strong antibody-antigen interactions.
When developing antibodies, many features can be required such as functionality (inhibiting activities for instance), specificity, use in particular applications and of course affinity. But it can be tricky to select an antibody with all these features from the start. It is therefore relevant to first focus on the functionality and application before working on improving the affinity using our high-class phage display platform.
For more information or to get a quote for our phage display services with the use of Affinity Maturation, don’t hesitate to contact us.