XtenCHO ™ Transfection kit

Reference: PX-XTE-003
Expression systemEukaryotic expression
Delivery conditionDry Ice
Delivery lead time in business daysEurope: 5-7 business days
USA & Canada: 7-10 business days
Rest of the world: 5-12 business days
Storage condition-20°C
Host speciesMammalian cells
ReferencePX-XTE-003
Publications
  • Luo K, Chen Y, Wang F. Shrimp Plasma MANF Works as an Invertebrate Anti-Inflammatory Factor via a Conserved Receptor Tyrosine Phosphatase. J Immunol. 2022 Mar 1;208(5):1214-1223. doi: 10.4049/jimmunol.2100595. Epub 2022 Feb 11. PMID: 35149533.
  • Bonduelle O, Chaudesaigues C, Tolazzi M, Suleiman E, de Bernard S, Alves K, Nourikyan J, Bohec M, Baudrin LG, Katinger D, Debré P, Scarlatti G, Vieillard V, Combadière B. Dichotomy in Neutralizing Antibody Induction to Peptide-Conjugated Vaccine in Squalene Emulsion Contrast With Aluminum Hydroxide Formulation. Front Immunol. 2022 Apr 7;13:848571. doi: 10.3389/fimmu.2022.848571. PMID: 35464449; PMCID: PMC9021396.
  • Sandberg, A., Berenjeno-Correa, E., Rodriguez, R.C. et al. Aβ42 oligomer-specific antibody ALZ-201 reduces the neurotoxicity of Alzheimer’s disease brain extracts. Alz Res Therapy 14, 196 (2022). https://doi.org/10.1186/s13195-022-01141-1
  • Villatoro, A., Cuminetti, V., Bernal, A. et al. Endogenous IL-1 receptor antagonist restricts healthy and malignant myeloproliferation. Nat Commun 14, 12 (2023). https://doi.org/10.1038/s41467-022-35700-9
NoteFor research use only
Kit Content
  • XtenFect Reagent, Stock solution: 1 microtube stock solution of 0.51 ml
  • XtenCHO™ Enhancer: 3 microtubes of 1.2 ml
  • XtenFect Reagent, Working solution: 3 microtubes of 1.44 ml
Limited Use Label LicensesLimited Use Label License for internal Research and Development Use of Mammalian Cell Lines Notice to Purchaser: The purchase of this product conveys to the purchaser the limited, non-transferable right to use the purchased amount of the product and progeny, to perform internal research and development for the sole benefit of the purchaser. The purchase of this product does not grant the purchaser any additional rights, including, without limitation: (a) the right to use the product or its components in commercial applications of any kind, including bioproduction (e.g. use of the product to manufacture therapeutic agents or diagnostic test components, such as proteins, antibodies, viral particles or virus-like particles), quality control and commercial services such as reporting the results of purchaser’s activities for a fee or other form of consideration; (b) the right to use the product or its components as a therapeutic agent or diagnostic test component; (c) the right to use the product or its components to produce material for use in human clinical trials; or (d) the right to transfer or resell the product or its components in any form, progeny or derivative. For information on obtaining additional rights, please contact us at contact@proteogenix.fr, Licensing and Commercial Supply, ProteoGenix, 19 rue de La Haye 67300 Schiltigheim, France. TRADEMARKS: All trademarks are the property of ProteoGenix and its subsidiaries unless otherwise specified.

Transfection

Transfection is the key step of recombinant proteins production in eukaryotic cells. The aim of this technique is to inject nucleic acid strains into the cells. The coding material thus implemented will use the cell own machinery to express its proteins. This step is highly costing and dangerous to the cells. Therefore, different technologies have been developed to enable the researchers to achieve it succefully. Proteogenix has engineered its own proprietary cell line XtenCHO™ to use it for its recombinant productions. It is now available to its customers. The XtenCHO™ Transfection Kit has been specifically optimized to support the transient transfection of XtenCHO™ Cells. Thanks to this tool the user can benefit from the unprecedented high yields of this cell line.

XtenCHO™ Transfection Kit content

The XtenCHO™ Transfection Kit is composed of XtenFect Reagent and the XtenCHO™ Enhancer. The XtenFect Reagent is optimized for the transfection of XtenCHO™ cell cultures grown in XtenCHO™ Expression Medium. It enables the Chinese Hamster Ovarian (CHO) cells to efficiently take on the nucleic acids. This step is critical to reach the highest recombinant protein production yields with XtenCHO™ Expression System. The XtenCHO™ Enhancer is optimized to maintain high viability in high density transfected XtenCHO™ Cells. This leads to extended production time and higher yields per liter of culture.
The XtenFect Reagent is provided as a 10 times concentrated stock solution that has to be diluted in the provided XtenFect Working Solution. After being reconstituted, the XtenFect working solution can be directly added to the XtenCHO™ cell culture. On the contrary to other transfection reagents, it does not need a previous step of complexe formation with DNA, making the process easier and faster. This product is intended for 1L of culture. These products are animal component free and chemically composed.
The XtenCHO™ Transfection Kit is a component of the XtenCHO™ Starter Kit.

Up to 10x higher yields without supplemental feeds

Therapeutic antibodies from several IgG subclasses and isotypes (IgG1-kappa and lambda, IgG2-lambda, IgG4-kappa) were produced with XtenCHO ™ cells and in parallel with CHO-S ™ and ExpiCHO ™ expression systems. XtenCHO ™ cells were transfected with ProteoGenix' proprietary TGE system (transfection protocol, transfection reagent and culture medium). CHO-S ™and ExpiCHO ™ cells were transfected according to manufacturer's instructions for each expression system (protocol, reagents and culture medium). Determination of production yields was done with an Octet Red96 system (Protein G biosensor).

Publication

  • Luo K, Chen Y, Wang F. Shrimp Plasma MANF Works as an Invertebrate Anti-Inflammatory Factor via a Conserved Receptor Tyrosine Phosphatase. J Immunol. 2022 Mar 1;208(5):1214-1223. doi: 10.4049/jimmunol.2100595. Epub 2022 Feb 11. PMID: 35149533.
  • Bonduelle O, Chaudesaigues C, Tolazzi M, Suleiman E, de Bernard S, Alves K, Nourikyan J, Bohec M, Baudrin LG, Katinger D, Debré P, Scarlatti G, Vieillard V, Combadière B. Dichotomy in Neutralizing Antibody Induction to Peptide-Conjugated Vaccine in Squalene Emulsion Contrast With Aluminum Hydroxide Formulation. Front Immunol. 2022 Apr 7;13:848571. doi: 10.3389/fimmu.2022.848571. PMID: 35464449; PMCID: PMC9021396.
  • Sandberg, A., Berenjeno-Correa, E., Rodriguez, R.C. et al. Aβ42 oligomer-specific antibody ALZ-201 reduces the neurotoxicity of Alzheimer’s disease brain extracts. Alz Res Therapy 14, 196 (2022). https://doi.org/10.1186/s13195-022-01141-1
  • Villatoro, A., Cuminetti, V., Bernal, A. et al. Endogenous IL-1 receptor antagonist restricts healthy and malignant myeloproliferation. Nat Commun 14, 12 (2023). https://doi.org/10.1038/s41467-022-35700-9

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