Entrust experienced professionals for antibody sequencing. Getting your monoclonal antibody sequence is a key starting point :
Our prices are among the most competitive on the market!
No sequence no fee
If we do not succeed in determining the sequence? You don’t pay!
Benefit from a fast service thanks to our standardized process!
Monoclonal antibody development expert
Increase your success rate by using our engineering and production services!
Get an antibody sequencing service strictly adapted to your requirements!
One stop solution
From early antibody discovery to the clinic!
Hybridoma cell line delivery
DNA sequencing of at least 5 samples for sequence alignment
Sub-cloning into a standard vector
Data analysis and report writing
Optional: test expression + antibody/antigen interaction test
ProteoGenix offers a fully customizable antibody sequencing service with high guarantees. With 16 years of experience, our services are exhaustive and standardized and featured as follow for optimal performances:
Here is an example of a customer requesting to sequence a hybridoma cell line we had previously developed for him.
Total RNA was extracted from hybridoma cells and cDNA subsequently synthesized. Antibody variable genes were then amplified by isotype-specific PCR, separately sub-cloned into a standard cloning vector and sequenced.
Antibody isotype determination is performed by gel electrophoresis. Details of the protocol are described in the complete report provided in PDF file.
Results of the sequencing are based on the analysis of at least 10 positive clones and include:
After completion of the antibody sequencing work, we were asked to engineer a chimeric version, synthesize both antibody cDNAs, subclone them into an expression vector, produce them in XtenCHO cells and determine antibody binding affinity (KD determination) of all 3 antibodies. The aim was to confirm that the chimeric recombinant antibody functionality at least remained equivalent to the mouse recombinant antibody and the mouse hybridoma antibody.
After confirmation, we were then able to develop humanized antibodies as well as bispecific antibodies for human therapeutic purposes.
Click on the button below to get the complete report “Parental Antibody Sequencing from Hybridoma”
With more than 15 years of experience in antibody development and production, ProteoGenix can offer you far more than your hybridoma sequence. Using our antibody sequencing service can be the start of a long and fruitful collaboration that will significantly increase your chance to go to the clinic. ProteoGenix offers a one-stop solution tailored to take your project further, such as:
Therapeutic Antibody Development
ProteoGenix offers high-quality engineering solutions to optimize the properties of your therapeutic antibodies including antibody humanization, affinity maturation, bispecific antibodies, antibody drug conjugates etc..
Recombinant Antibody Production
ProteoGenix provides one of the highest performing recombinant antibody production platforms in the world thanks to 16 years of experience and proprietary technologies like XtenCHOTM.
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