ProteoGenix has overcome some challenges to be able to offer a unique rabbit monoclonal antibody production service using a premium antibody library and an optimized platform of antibody phage display for diagnostic applications. These antibodies are particularly adapted to diagnostics due to their enhanced specificity and selectivity, reducing off-target binding and maximizing the success of your projects.
Rabbit monoclonal antibodies are a class of reagents with very high added value for research, diagnostic and therapeutic applications.
They naturally have:
Rabbit monoclonal antibodies are mostly derived from hybridomas, which are generally not well characterized and which sometimes show high batch-to-batch variability.
The hybridoma method screening is very limited (~103), often it misses rare clones and takes a while to produce antibodies (approximately 9 months). Additionally, clones are often very unstable and antibody production from them can be troublesome. In contrast, phage display combines the superior antigen recognition of the rabbit immune system with the specificity and consistency of a monoclonal antibody generated via hybridoma development without the drawbacks of rabbit hybridomas.
Based on their binding affinity and specificity, phage display is one of the most robust technologies used for large-scale studies and the selection of proteins, peptide and antibodies. We believe that monoclonal antibody production in rabbit using phage display offers many advantages compared to other technologies:
Faster and cost effective
We can build phage display libraries very quickly. Our technology will radically reduce the time (3 – 5 months) and expenses for your project
Higher screening range
(107– 109)
Abundance
Once we have created libraries, we can reproduce them promptly in phage and use them for an unlimited number of screenings
Enhance the specificity and stability
with which antibodies recognize antigens in various environmental conditions
Higher production
Of antibody clones at a time
No fusion
No stability issues of rabbit hybridomas and availability of the antibody sequence to secure it forever
Animal protection
As phage display (from naive libraries) doesn’t require any animal use
In this case we offer two possible ways to start the project by phage display:
One method is based on a naive library (coming soon), all the genes of antibodies collected from bone marrow, blood and other samples are included in the library and are suitable for selecting antibodies that match with all kinds of antigens. If you are requesting an immunization with a higher chance of getting the best binders, we offer you the possibility of building an immune library.
Below are the timeframes for the screening of both libraries:
Starting with screening a naive library Takes about 6-7 weeks (no immunization needed)
Starting with construction and screening an immune library Takes about 15-18 + 6-7 weeks (including immunization)
Immunization of 3-5 aninimals
Phage display library constrution
Biopanning: screening, amplification and identification
Rabbit Monoclonal Antibody production: Validation by ELISA
For more information or to get a quote for our service, don’t hesitate to contact us.
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