Interested in implementing the use of VHH single domain antibodies in your diagnostic applications? At ProteoGenix, we have developed an optimized platform of antibody phage display for diagnostic applications. Target cryptic epitopes and enhance antibody tissue diffusion by choosing our services with unique guarantees.

ProteoGenix offers VHH single domain antibody production.

Our service includes:

  • The construction of an immune library using our alpacas/llamas or the use of
    our naive library
  • Bio-panning of naive or immune libraries via phage display technology
  • Large scale production of VHH single domain antibody
  • Library construction and phage display screening

What is a VHH single domain antibody?

Conventionally we use IgG antibodies from mice, rat, rabbit etc. They have a size of 150kDa and are composed of 2 heavy and 2 light chains.

A group of researcher found that all members of the Camelidae family possess a special subclass IgG devoid of light chains; these antibodies consist of two heavy chains attached to variable domains (VHH).
Due to this, camelidae antibodies are much smaller, about 95kDa. The VHH alone, which is 15kDa, is also known as single domain antibody.
It is considered as the smallest naturally derived antigen-binding fragment, which is important for the development of several antibody drugs or diagnostic tools.

Conventional antibody VS Camelidae antibody : Camelidae antibody doesn't have light chain and have on its heavy chain VHH single domain antibody
Conventional antibody VS Camelidae antibody

Advantages using VHH single domain antibody

Small size

The smaller size of VHH single domain antibodies gives them the ability to fit into “hidden” epitopes that are usually not accessible to conventional antibodies, including active site of many enzymes

Highly stable

VHH single domain antibodies are highly stable and resistant to acid solution and high temperatures, they are able to fold back into a functional protein after denaturation

Highly soluble

VHH single domain antibodies are highly soluble due to their increased hydrophilic function

The main advantage of VHH single domain antibodies is that they have outstanding binding properties and can be expressed at at consistently high quality without batch-to-batch variation.
Due to their high affinity and binding specificities, these types of antibodies are robust tools for a lot of bioassays and applications.

VHH single domain antibody production steps

Immuniztion of our alpages, llamas or cameis (not needed if using an naive library)

Phage display library construction

Biopanning: screening amplification and hit identifiaction

VHH single domin antibody production & analytical tests (ELISA, Biacore, SEC-HPLC, DSC)

Featured VHH single domain antibody applications

There are a lot of applications, described in scientific publications, that use VHH single domain antibodies. This is due to their smaller size which leads to better tissue penetration. You can find detailed information for:

  • Therapeutic applications.
  • Crystallography applications.
  • Biotechnological applications.
  • Diagnostics applications etc.


We believe that VHH single domain antibodies are a good choice for a lot of applications and assays.

For more information or to get a quote for our service, don’t hesitate to contact us.