Lymphoprep™ Tube

Datasheet of Lymphoprep™ Tube

Introduction

Lymphoprep™ Tube is a specialized cell separation media that is widely used in biological research and clinical applications. It is a sterile, isotonic solution that is designed for the isolation of lymphocytes from whole blood or other biological samples. This unique media is composed of a mixture of polysaccharides and inorganic salts, which creates a density gradient that allows for the separation of different cell types based on their density. In this article, we will explore the structure, activity, and applications of Lymphoprep™ Tube in detail.

Structure of Lymphoprep™ Tube

Lymphoprep™ Tube is a clear, colorless solution that is supplied in a sterile, single-use tube. The tube contains a mixture of polysaccharides, including ficoll and sodium metrizoate, and inorganic salts such as sodium chloride and sodium diatrizoate. These components are carefully selected to create a density gradient that is essential for the separation of lymphocytes from other cells.

Activity of this cell separation medium

The activity of Lymphoprep™ Tube is based on the principle of density gradient centrifugation. When a biological sample, such as whole blood, is added to the tube, the different cell types in the sample will settle at different levels in the density gradient based on their density. Lymphocytes, which are less dense than other cells in the sample, will float to the top of the gradient, while heavier cells, such as red blood cells and granulocytes, will settle at the bottom.

The density gradient is created by the polysaccharides and inorganic salts in the tube. These components form a viscous solution with a specific density that is higher than that of the sample. As the tube is centrifuged, the sample will pass through the density gradient and separate into distinct layers based on their density. The resulting layer at the top of the gradient will contain highly purified lymphocytes, which can then be collected for further analysis.

Applications of Lymphoprep™ Tube

Lymphoprep™ Tube has a wide range of applications in biological research and clinical settings. Some of the common applications include:

1. Isolation of lymphocytes for immune cell studies: Lymphoprep™ Tube is commonly used to isolate lymphocytes from whole blood or other biological samples for immune cell studies. The highly purified lymphocytes obtained from this process are essential for studying the immune response to various diseases and infections.

2. Separation of mononuclear cells for stem cell research: Mononuclear cells, which include lymphocytes, monocytes, and stem cells, can be separated using Lymphoprep™ Tube. This technique is particularly useful in stem cell research, where the purity of the cell population is crucial for successful experiments.

3. Preparing lymphocytes for flow cytometry analysis: Flow cytometry is a powerful technique used to analyze and sort different types of cells based on their physical and chemical properties. Lymphoprep™ Tube is often used to isolate lymphocytes for flow cytometry analysis, as it provides a highly pure population of cells for accurate results.

4. Isolation of lymphocytes for diagnostic purposes: In clinical settings, Lymphoprep™ Tube is used to isolate lymphocytes from patient samples for diagnostic purposes. This technique is particularly useful in detecting and monitoring diseases that affect the immune system, such as leukemia and lymphoma.

Conclusion

Lymphoprep™ Tube is a highly effective cell separation media that is widely used in biological research and clinical applications. Its unique structure and activity make it an essential tool for isolating lymphocytes and other mononuclear cells from various biological samples. With its wide range of applications, Lymphoprep™ Tube continues to play a significant role in advancing our understanding of the immune system and its role in health and disease.