Storage and Stability Conditions of Polymorphprep™
• Polymorphprep™ should be stored at room temperature (20°C). • It’s stable for 3 years provided the solution is kept sterile and protected from light. • Prolonged exposure to direct sunlight leads to release of iodine from the Sodium Diatrizoate molecule (this effect is negligible when working with the solution on a day to day basis.)
Brand
Axis-Shield/Serumwerk
Composition
The solution contains Sodium Diatrizoate and Dextran 500 in the following concentrations: • Sodium diatrizoate 13.8% (w/v) • Dextran 500 8.0 (w/v)
Principle of the separation procedure
How Polymorphprep™ works
Polymorphonuclear leukocytes (PMNs) has a lower buoyant density than erythrocytes (1.09-1.11 g/ml) but a higher buoyant density than the mononuclear cells (higher than 1.085 g/ml). Therefore, it is difficult to isolate polymorphonuclear granulocytes using a density barrier similar to that used for mononuclear cell. Several procedures have been developed to overcomes these issues. The high osmolality of Polymorphprep™ causes erythrocytes to lose water and shrink, thus increasing their effective buoyant densities. This allows the dextran aggregated erythrocytes to sediment rapidly through the dense medium. As the cells sediment further into the medium, the osmotic gradient between the medium and the erythrocytes declines. This means that water loss from the erythrocytes is greater at the top of the Polymorphprep™ and progressively decreases as they sediment further. A gradient of diatrizoate is formed within the density barrier. The PMNs band within this density gradient while the mononuclear cells remain at the sample/medium interface.
Advantages
Polymorphprep™ vs Ficoll-Paque
• Ficoll-Paque (GE Health Care) is not endotoxin tested at a base level and higher price • Unlike Polymorphprep, Ficoll-Paque is not manufactured under EU cGMP compliance.
Alternatives
Polymorphprep™ can be a good substitute for Ficoll-Paque
Reference
1114683
Description of Polymorphprep™
Polymorphprep™ is a sterile density gradient medium designed for the isolation of polymorphonuclear leukocytes (PMNs), including neutrophils, from human peripheral blood. The optimized density formulation allows efficient separation of granulocytes from erythrocytes and mononuclear cells using standard centrifugation procedures.
This ready-to-use reagent enables laboratories to obtain highly purified neutrophil populations suitable for a wide range of downstream research applications. Polymorphprep™ provides reliable and reproducible cell separation while preserving cell integrity and functionality.
Polymorphprep™ is widely used in immunology, inflammation, and cell biology research, where purified polymorphonuclear cells are required for functional assays, cell analysis, and experimental studies.
Manufactured under strict quality standards, Polymorphprep™ ensures consistent performance and high reproducibility across experiments.
For research use only. Not for diagnostic or therapeutic use.
Key Features
Density gradient medium for neutrophil isolation
Efficient separation of polymorphonuclear cells and erythrocytes
Ready-to-use formulation
Reliable and reproducible cell separation
Suitable for research laboratories
Applications
Isolation of human neutrophils
Preparation of polymorphonuclear leukocytes (PMNs)
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