Obinutuzumab ELISA Kit

Description of Obinutuzumab ELISA Kit

Structure of Obinutuzumab ELISA Kit

The Obinutuzumab ELISA Kit is a highly sensitive and specific assay designed for the quantitative measurement of Obinutuzumab, a monoclonal antibody, in biological samples. The kit utilizes a sandwich ELISA format, where the target antigen is captured between two specific antibodies, one coated on the microplate and the other conjugated to an enzyme. The kit is composed of a microplate coated with a monoclonal antibody specific for Obinutuzumab, a detection antibody conjugated to horseradish peroxidase (HRP), and all necessary reagents for the assay.
Obinutuzumab is a humanized IgG1 monoclonal antibody that targets the CD20 antigen, which is expressed on the surface of B-cells. The antibody is composed of two heavy chains and two light chains, and has a molecular weight of approximately 150 kDa. The heavy chain consists of four constant domains (CH1, CH2, CH3, and CH4) and one variable domain (VH), while the light chain has two constant domains (CL and CL2) and one variable domain (VL). The variable domains of the antibody are responsible for binding to the CD20 antigen, while the constant domains provide structural stability and effector functions.

Activity of Obinutuzumab ELISA Kit

The Obinutuzumab ELISA Kit is a highly sensitive and specific assay that can quantitatively measure Obinutuzumab in various biological samples, such as serum, plasma, and cell culture supernatant. The assay utilizes the principle of sandwich ELISA, where the target antigen is captured between two specific antibodies. In this case, the microplate is coated with a monoclonal antibody specific for Obinutuzumab, while the detection antibody is conjugated to HRP. The HRP enzyme catalyzes a colorimetric reaction, which can be measured spectrophotometrically and is directly proportional to the amount of Obinutuzumab present in the sample.
The Obinutuzumab ELISA Kit has a wide dynamic range of detection, with a lower limit of quantification (LLOQ) of 0.1 ng/mL and an upper limit of quantification (ULOQ) of 10 ng/mL. The assay has a high sensitivity, with an inter-assay coefficient of variation (CV) of less than 10% and an intra-assay CV of less than 8%. The kit also has a high specificity, as it does not cross-react with other monoclonal antibodies or endogenous proteins.

Application of Obinutuzumab ELISA Kit

The Obinutuzumab ELISA Kit has a wide range of applications in the field of research and clinical diagnostics. It can be used to measure the levels of Obinutuzumab in patient samples, which can be helpful in monitoring the efficacy of treatment and predicting response to therapy. The kit can also be used in preclinical studies to determine the pharmacokinetics and pharmacodynamics of Obinutuzumab, as well as in drug development and quality control.
Furthermore, the Obinutuzumab ELISA Kit can be used in basic research to study the mechanism of action of Obinutuzumab and its interactions with the CD20 antigen. It can also be used to measure the levels of Obinutuzumab in cell culture supernatant, which can be helpful in optimizing production processes and evaluating the stability of the antibody.
In conclusion, the Obinutuzumab ELISA Kit is a highly sensitive and specific assay that provides a quantitative measurement of Obinutuzumab in various biological samples. Its wide range of applications makes it a valuable tool for both research and clinical purposes, and its high sensitivity and specificity make it a reliable choice for accurate and reproducible results.