Recombinant Human ABCF1, N-His

Description of Recombinant Human ABCF1, N-His

Introduction to Recombinant Human ABCF1

Recombinant human ABCF1 is a protein that plays a crucial role in cellular processes such as RNA metabolism and translation. It is a member of the ATP-binding cassette (ABC) protein family, which is known for its involvement in the transport of various molecules across cell membranes. The ABCF1 gene is located on chromosome 6 and encodes for a 67 kDa protein with 616 amino acids. In this article, we will discuss the structure, activity, and applications of recombinant human ABCF1.

Structure of Recombinant Human ABCF1

The structure of recombinant human ABCF1 is composed of two main domains, the N-terminal ATP-binding domain and the C-terminal nucleotide-binding domain. These domains are connected by a linker region and are responsible for the ATPase activity of the protein. The N-terminal domain contains the Walker A and B motifs, which are involved in ATP binding and hydrolysis. The C-terminal domain contains the signature sequence of the ABC protein family, the ABC signature motif, which is essential for the transport function of the protein.

In addition to these domains, recombinant human ABCF1 also contains a highly conserved histidine-rich region, which is believed to play a role in RNA binding. This region is located between the two ATP-binding domains and is highly conserved in all ABCF1 proteins across different species.

Activity of Recombinant Human ABCF1

The main activity of recombinant human ABCF1 is its involvement in the regulation of RNA metabolism. It has been shown to interact with various RNA-binding proteins, such as eIF4G and PABP, and play a role in the formation of the eIF4F complex, which is essential for translation initiation. Additionally, ABCF1 has been found to regulate the translation of specific mRNAs by binding to their 5′ untranslated regions (UTRs).

Furthermore, recombinant human ABCF1 has been shown to have ATPase activity, which is essential for its function in RNA metabolism. This activity is regulated by the binding of ATP and the presence of RNA, suggesting a potential role in energy-dependent RNA processing and translation.

Applications of Recombinant Human ABCF1

Recombinant human ABCF1 has a wide range of applications in both basic research and clinical settings. Its role in RNA metabolism and translation makes it a potential target for therapeutic interventions in diseases such as cancer and viral infections.

One of the most significant applications of recombinant human ABCF1 is in the production of recombinant proteins. The ATPase activity of ABCF1 can be utilized to aid in the proper folding and processing of recombinant proteins, leading to increased yields and improved protein quality.

Moreover, recombinant human ABCF1 has been used as an antigen in vaccine development for viral infections such as hepatitis C and HIV. Its ability to bind to specific RNA sequences and regulate translation makes it an attractive target for vaccine design.

Additionally, recombinant human ABCF1 has been studied for its potential role in cancer progression and metastasis. It has been found to be overexpressed in various cancers and has been linked to increased cell proliferation and invasion. Targeting ABCF1 in cancer cells could potentially lead to the development of novel anti-cancer therapies.

Conclusion

In conclusion, recombinant human ABCF1 is a multifunctional protein with a crucial role in RNA metabolism and translation. Its structure, activity, and various applications make it a valuable tool in both basic research and clinical settings. Further studies on this protein could provide valuable insights into its role in disease and potential therapeutic interventions.

Keywords: recombinant protein, antigen, RNA metabolism, translation, ATPase activity, vaccine, cancer, therapeutic interventions