Recombinant Human CAMK2G Protein, N-His

Description of Recombinant Human CAMK2G Protein, N-His

Introduction

Recombinant Human CAMK2G Protein, also known as Calcium/calmodulin-dependent protein kinase type II subunit gamma, is a protein that plays a crucial role in regulating various cellular processes. It is a member of the CAMK2 family of serine/threonine kinases and is encoded by the CAMK2G gene.

Structure of Recombinant Human CAMK2G Protein

The recombinant protein is a 52 kDa polypeptide consisting of 478 amino acids. It has a conserved domain structure with a catalytic domain at the N-terminus and a regulatory domain at the C-terminus. The catalytic domain contains the ATP-binding site and the catalytic loop responsible for the protein kinase activity. The regulatory domain contains the autophosphorylation site and the calmodulin-binding site, which regulates the activity of the protein.

Activity of Recombinant Human CAMK2G Protein

Recombinant Human CAMK2G Protein is a multifunctional enzyme that is involved in various cellular processes such as neuronal signaling, synaptic plasticity, and gene expression. It is activated by the binding of calcium/calmodulin, which induces a conformational change in the protein, leading to its activation. Once activated, it phosphorylates a wide range of target proteins, thereby regulating their activity and function.

One of the key functions of CAMK2G is its role in neuronal signaling. It is highly expressed in the brain and is essential for learning and memory. It regulates the activity of ion channels and neurotransmitter receptors, thereby modulating synaptic transmission and plasticity. It also plays a crucial role in the formation and maintenance of long-term potentiation (LTP), a process that is essential for learning and memory.

In addition to its role in neuronal signaling, recombinant CAMK2G protein also plays a role in gene expression. It has been shown to phosphorylate various transcription factors, such as CREB and Elk-1, which regulate the expression of genes involved in neuronal development and plasticity. It also regulates the activity of histone deacetylases, which play a crucial role in epigenetic regulation of gene expression.

Application of Recombinant Human CAMK2G Protein

Recombinant Human CAMK2G Protein has a wide range of applications in both basic research and drug development. Its role in neuronal signaling and plasticity makes it a valuable tool for studying learning and memory processes. It can be used to investigate the molecular mechanisms underlying neurological disorders, such as Alzheimer’s disease and schizophrenia.

Furthermore, recombinant CAMK2G protein has potential therapeutic applications. It has been shown to play a role in the development and progression of various diseases, including cancer and cardiovascular diseases. Therefore, targeting CAMK2G activity could be a potential strategy for the treatment of these diseases. Recombinant CAMK2G protein can also be used for drug screening and development, as it is a potential drug target for various diseases.

Conclusion

Recombinant Human CAMK2G Protein is a crucial enzyme that plays a vital role in regulating various cellular processes. Its structure, activity, and applications make it a valuable tool for both basic research and drug development. Further studies on this protein could provide valuable insights into its role in health and disease and could lead to the development of new therapeutic strategies.