Recombinant Human CHFR Protein, N-His

Description of Recombinant Human CHFR Protein, N-His

Introduction to Recombinant Human CHFR Protein

Recombinant Human CHFR Protein, also known as checkpoint protein CHFR, is a type of protein that plays a crucial role in the regulation of cell cycle and DNA damage response. This protein is encoded by the CHFR gene and is found in humans. Recombinant Human CHFR Protein is produced through recombinant DNA technology, making it a valuable tool in various scientific and medical applications.

Structure of Recombinant Human CHFR Protein

The structure of Recombinant Human CHFR Protein consists of 740 amino acids and has a molecular weight of approximately 85 kDa. It belongs to the RING finger protein family and contains a RING finger domain, a forkhead-associated (FHA) domain, and a C-terminal region. The RING finger domain is responsible for the E3 ubiquitin ligase activity of this protein, while the FHA domain is involved in protein-protein interactions.

Recombinant Human CHFR Protein also contains a nuclear localization signal (NLS) and a nuclear export signal (NES), allowing it to shuttle between the nucleus and cytoplasm. This protein is predominantly found in the cytoplasm, but can translocate to the nucleus in response to DNA damage.

Activity of Recombinant Human CHFR Protein

The main function of Recombinant Human CHFR Protein is to act as a checkpoint protein in the cell cycle. It is involved in the G2/M checkpoint, which ensures that cells have repaired any DNA damage before proceeding to cell division. This protein is activated in response to DNA damage and acts as a tumor suppressor by preventing the proliferation of cells with damaged DNA.

Recombinant Human CHFR Protein has E3 ubiquitin ligase activity, which means it can attach ubiquitin molecules to target proteins. This activity is crucial for its role in the cell cycle, as it can target and degrade proteins involved in cell cycle progression, such as cyclin B1 and Cdc25C. By doing so, it delays cell cycle progression and allows time for DNA repair to occur.

Application of Recombinant Human CHFR Protein

Recombinant Human CHFR Protein has various applications in the field of cancer research and drug development. Its role as a tumor suppressor makes it a potential target for cancer therapy. Studies have shown that mutations in the CHFR gene are associated with various types of cancer, including breast, ovarian, and lung cancer. Therefore, understanding the function of this protein can provide insights into the development and progression of these cancers.

Another potential application of Recombinant Human CHFR Protein is in the development of diagnostic tools. As this protein is activated in response to DNA damage, its levels can be used as a biomarker for DNA damage and cancer. This could aid in the early detection and monitoring of cancer.

Recombinant Human CHFR Protein can also be used in drug screening assays to identify compounds that can modulate its activity. By targeting this protein, it may be possible to sensitize cancer cells to DNA damage and increase their susceptibility to chemotherapy.

Conclusion

In summary, Recombinant Human CHFR Protein is a crucial protein involved in the regulation of cell cycle and DNA damage response. Its structure, activity, and applications make it a valuable tool in various scientific and medical fields, particularly in cancer research and drug development. Further studies on this protein may provide more insights into its function and potential therapeutic applications.