Recombinant Human CTBP1 Protein, N-His

Description of Recombinant Human CTBP1 Protein, N-His

Introduction

Recombinant human CTBP1 protein, also known as C-terminal binding protein 1, is a highly conserved transcriptional co-repressor that plays a crucial role in regulating gene expression. This protein is encoded by the CTBP1 gene and is found in various tissues and cell types, including the brain, heart, and immune cells. In this article, we will discuss the structure, activity, and applications of recombinant human CTBP1 protein.

Structure of Recombinant Human CTBP1 Protein

Recombinant human CTBP1 protein is a 48 kDa protein that consists of 445 amino acids. It contains two NAD(H)-binding domains, a proline-rich region, and a C-terminal domain. The NAD(H)-binding domains are responsible for binding to NAD(H), which is essential for the protein’s activity. The proline-rich region is involved in protein-protein interactions, while the C-terminal domain is responsible for DNA binding and transcriptional repression.

Activity of Recombinant Human CTBP1 Protein

Recombinant human CTBP1 protein acts as a transcriptional co-repressor by interacting with various transcription factors, such as zinc finger proteins, to inhibit gene expression. It does so by recruiting histone deacetylases (HDACs) to the target gene’s promoter region, leading to the formation of a repressive chromatin structure. This results in the suppression of gene transcription and regulation of various cellular processes, including cell proliferation, differentiation, and apoptosis.

Additionally, recombinant human CTBP1 protein has been shown to play a role in cellular metabolism. It binds to NAD(H) and acts as a regulator of the NAD(H) pool, which is essential for cellular energy production. It also interacts with enzymes involved in glycolysis and regulates their activity, thus impacting cellular metabolism.

Applications of Recombinant Human CTBP1 Protein

Recombinant human CTBP1 protein has various applications in both research and therapeutic settings. Its ability to act as a transcriptional co-repressor makes it a valuable tool for studying gene expression and regulation. It can be used to investigate the role of CTBP1 in different cellular processes and pathways, as well as its interactions with other transcription factors.

In addition, recombinant human CTBP1 protein has potential therapeutic applications. It has been found to be overexpressed in various types of cancer, including breast, colon, and lung cancer. Inhibition of CTBP1 activity has been shown to suppress tumor growth and sensitize cancer cells to chemotherapy, making it a potential target for cancer treatment.

Moreover, recombinant human CTBP1 protein has been studied for its role in metabolic disorders, such as diabetes. It has been shown to regulate insulin secretion and glucose metabolism in pancreatic beta cells, making it a potential target for the treatment of diabetes.

Conclusion

In summary, recombinant human CTBP1 protein is a crucial transcriptional co-repressor that plays a role in gene expression and cellular metabolism. Its structure, activity, and applications make it a valuable tool in research and potential target for therapeutic interventions. Further studies on this protein may provide valuable insights into its role in various diseases and potential treatment options.