Recombinant Human E2F1/RBBP3 Protein, N-His

Description of Recombinant Human E2F1/RBBP3 Protein, N-His

Introduction

Recombinant Human E2F1/RBBP3 Protein is a highly active and versatile protein that plays a crucial role in cell cycle regulation and DNA repair. It is a fusion protein consisting of two essential components, E2F1 and RBBP3, which work together to regulate gene expression and promote cell cycle progression. In this article, we will discuss the structure, activity, and various applications of this recombinant protein.

Structure of Recombinant Human E2F1/RBBP3 Protein

The recombinant protein is produced by cloning the genes for E2F1 and RBBP3 into an expression vector and then expressing it in a suitable host cell. The resulting protein is a fusion of E2F1 and RBBP3, with a molecular weight of approximately 60 kDa. It contains all the functional domains of both proteins, including the DNA-binding domain of E2F1 and the histone-binding domain of RBBP3.

The recombinant protein has a unique structure that allows it to interact with multiple proteins and regulate various cellular processes. It forms a stable complex with RBBP3, which helps in its proper folding and stability. This complex also plays a crucial role in the recruitment of other proteins involved in cell cycle regulation and DNA repair.

Activity of Recombinant Human E2F1/RBBP3 Protein

Recombinant Human E2F1/RBBP3 Protein has multiple activities that make it an essential player in cell cycle regulation and DNA repair. Its primary function is to regulate the transcription of genes involved in cell cycle progression, DNA replication, and repair. It binds to specific DNA sequences and recruits other proteins to promote or inhibit gene expression, depending on the cellular context.

In addition to its role in gene expression, the recombinant protein also plays a vital role in DNA repair. It interacts with various repair proteins, such as BRCA1 and RAD51, and helps in the recognition and repair of damaged DNA. It also plays a role in maintaining genome stability by preventing the accumulation of mutations and chromosomal abnormalities.

Applications of Recombinant Human E2F1/RBBP3 Protein

Recombinant Human E2F1/RBBP3 Protein has a wide range of applications in both basic research and clinical settings. Its ability to regulate gene expression and participate in DNA repair makes it a valuable tool for studying various cellular processes. It is commonly used in cell culture studies to investigate the effects of E2F1 and RBBP3 on cell cycle progression and DNA repair.

The recombinant protein also has potential clinical applications. It has been shown to have anti-tumor activity in various cancer cell lines, making it a potential therapeutic target for cancer treatment. It has also been implicated in the development of drug resistance in cancer cells, making it a potential biomarker for predicting treatment response.

Moreover, the recombinant protein has been used in diagnostic assays for various diseases, including cancer. Its ability to interact with specific DNA sequences makes it a valuable tool for detecting mutations and chromosomal abnormalities that are associated with certain diseases.

Conclusion

Recombinant Human E2F1/RBBP3 Protein is a highly active and versatile protein that plays a crucial role in cell cycle regulation and DNA repair. Its unique structure and multiple activities make it an essential player in various cellular processes. With its wide range of applications in both basic research and clinical settings, this recombinant protein continues to be a valuable tool for understanding and treating various diseases.

Keywords:

recombinant protein, antigen, E2F1, RBBP3, cell cycle regulation, DNA repair, gene expression, transcription, DNA replication, genome stability, cancer treatment, drug resistance, diagnostic assays.