Recombinant Human EIF2B1 Protein, N-His

Description of Recombinant Human EIF2B1 Protein, N-His

Introduction

Recombinant human EIF2B1 protein (rHuEIF2B1) is a genetically engineered version of the human EIF2B1 protein, which plays a crucial role in the regulation of protein synthesis in the cell. This protein is part of the eukaryotic translation initiation factor 2B (EIF2B) complex, which is responsible for the initiation of protein synthesis in eukaryotic cells. Recombinant protein technology has allowed for the production of large quantities of rHuEIF2B1, which has been used in numerous research studies to understand its structure, activity, and potential applications.

Structure of Recombinant Human EIF2B1 Protein

The human EIF2B1 gene is located on chromosome 12 and encodes for a protein with a molecular weight of approximately 72 kDa. The recombinant version of this protein is produced by inserting the human EIF2B1 gene into a host cell, such as bacteria or yeast, which then produces the protein in large quantities. The rHuEIF2B1 protein has a similar structure to the native human protein, with a conserved N-terminal domain and a C-terminal domain that is responsible for binding to other proteins in the EIF2B complex.

Activity of Recombinant Human EIF2B1 Protein

The primary function of rHuEIF2B1 is to regulate the activity of the EIF2B complex, which is essential for the initiation of protein synthesis. This protein binds to the guanine nucleotide exchange factor (GEF) domain of the EIF2B complex, which then activates the complex and allows for the exchange of guanosine diphosphate (GDP) for guanosine triphosphate (GTP) on the eukaryotic translation initiation factor 2 (eIF2). This exchange of nucleotides is necessary for the initiation of protein synthesis and is regulated by the activity of rHuEIF2B1.

Application of Recombinant Human EIF2B1 Protein

Recombinant human EIF2B1 protein has been used in various research studies to understand its role in protein synthesis and its potential applications. Here are some specific applications of rHuEIF2B1:

1. Study of Neurological Diseases: Mutations in the human EIF2B1 gene have been linked to a rare neurological disorder called leukoencephalopathy with vanishing white matter (VWM). Recombinant human EIF2B1 protein has been used in research studies to understand the structural and functional consequences of these mutations and their role in the development of VWM.

2. Cancer Research: The EIF2B complex has been implicated in the development and progression of various types of cancer. Recombinant human EIF2B1 protein has been used in studies to investigate the role of this protein in cancer cell growth and survival, and its potential as a therapeutic target.

3. Drug Development: The activity of the EIF2B complex is regulated by various cellular signaling pathways, making it an attractive target for drug development. Recombinant human EIF2B1 protein has been used in high-throughput screening assays to identify potential inhibitors of the EIF2B complex, which could lead to the development of new treatments for diseases such as cancer and neurological disorders.

4. Vaccine Development: Recombinant proteins, including rHuEIF2B1, have been used as antigens in vaccine development. These proteins can elicit an immune response and can be used to develop vaccines against infectious diseases, such as malaria and HIV.

Conclusion

Recombinant human EIF2B1 protein is a crucial component of the EIF2B complex, which plays a vital role in the regulation of protein synthesis in eukaryotic cells. The production of rHuEIF2B1 using recombinant protein technology has allowed for its use in various research studies to understand its structure, activity, and potential applications. This protein has shown promise in the study of neurological diseases, cancer research, drug development, and vaccine development. Further research on rHuEIF2B1 could lead to a better understanding of its role in cellular processes and the development of new treatments for various diseases.