Recombinant Human FGD4/FRABP Protein, N-His

Reference: YHK82601
Product nameRecombinant Human FGD4/FRABP Protein, N-His
Origin speciesHuman
Expression systemProkaryotic expression
Molecular weight24.84 kDa
BufferLyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
FormLiquid
Delivery conditionDry Ice
Delivery lead time in business days3-5 days if in stock; 3-5 weeks if production needed
Storage condition4°C for short term (1 week), -20°C or -80°C for long term (avoid freezing/thawing cycles; addition of 20-40% glycerol improves cryoprotection)
BrandAntibodySystem
Host speciesEscherichia coli (E.coli)
Fragment TypeSer545-Glu742
Aliases /SynonymsActin filament-binding protein frabin, ZFYVE6, FYVE, RhoGEF and PH domain-containing protein 4, FGD1-related F-actin-binding protein, FGD4, FRABP, Zinc finger FYVE domain-containing protein 6
ReferenceYHK82601
NoteFor research use only.

Description of Recombinant Human FGD4/FRABP Protein, N-His

Introduction to Recombinant Human FGD4/FRABP Protein

Recombinant Human FGD4/FRABP Protein is a highly specialized protein that plays a crucial role in various cellular processes. It belongs to the family of FGD4 proteins, which are known for their involvement in cell signaling and cytoskeletal organization. This specific protein is also referred to as FRABP, which stands for FGD4-Related F-BAR Adaptor Protein.

Structure of Recombinant Human FGD4/FRABP Protein

The Recombinant Human FGD4/FRABP Protein is composed of 1107 amino acids and has a molecular weight of approximately 124 kDa. It contains an N-terminal F-BAR (Fer/CIP4 and Bin/Amphiphysin/Rvs) domain, which is responsible for its membrane-binding and curvature-inducing properties. This domain is followed by a coiled-coil region and a C-terminal region that contains multiple SH3 (Src Homology 3) domains. These SH3 domains are known to interact with other proteins and play a role in signal transduction.

Activity of Recombinant Human FGD4/FRABP Protein

The main function of Recombinant Human FGD4/FRABP Protein is to act as a scaffolding protein, bringing together different signaling molecules and regulating their activity. It is involved in the formation and maintenance of actin-based structures, such as filopodia and lamellipodia, which are essential for cell movement and shape. This protein also plays a role in endocytosis, the process of internalizing molecules and particles into the cell.

Research has shown that Recombinant Human FGD4/FRABP Protein is highly expressed in the nervous system, where it regulates the formation and maintenance of neuronal structures. It is also found in other tissues, including the heart, kidney, and liver, indicating its involvement in various physiological processes.

Application of Recombinant Human FGD4/FRABP Protein

The unique structure and activity of Recombinant Human FGD4/FRABP Protein make it a valuable tool for various applications in both research and medicine. Some of its key applications include:

1. Recombinant Protein Production

Recombinant Human FGD4/FRABP Protein can be produced in large quantities using recombinant DNA technology. This allows for the production of pure and highly active protein, which can be used for further studies and applications.

2. Biomarker for Neurological Disorders

Studies have shown that mutations in the FGD4 gene, which codes for the Recombinant Human FGD4/FRABP Protein, are associated with Charcot-Marie-Tooth disease (CMT), a neurological disorder that affects the peripheral nerves. Therefore, this protein can serve as a biomarker for the diagnosis and monitoring of CMT and other related disorders.

3. Drug Target for Cancer Therapy

The overexpression of Recombinant Human FGD4/FRABP Protein has been observed in various types of cancer, including breast, lung, and liver cancer. This protein has been identified as a potential drug target for cancer therapy, as its inhibition can disrupt the formation of actin-based structures and inhibit tumor growth and metastasis.

4. Research Tool for Cell Signaling Studies

Recombinant Human FGD4/FRABP Protein is a crucial component of the signaling pathways involved in cell movement and shape. Its recombinant form can be used in research studies to understand the mechanisms of these pathways and their dysregulation in various diseases.

5. Antigen for Antibody Production

The unique structure and activity of Recombinant Human FGD4/FRABP Protein make it an ideal antigen for the production of antibodies. These antibodies can be used in various techniques, such as western blotting and immunofluorescence

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