Recombinant Human HNRNPU Protein, N-His

Description of Recombinant Human HNRNPU Protein, N-His

Introduction
Recombinant Human HNRNPU Protein, also known as heterogeneous nuclear ribonucleoprotein U (HNRNPU), is a multi-functional protein that plays a crucial role in various cellular processes, including RNA processing, transcription regulation, and DNA damage response. This protein is encoded by the HNRNPU gene and is highly conserved in mammals, making it an essential component of many biological systems. In this article, we will discuss the structure, activity, and applications of Recombinant Human HNRNPU Protein.

Structure of Recombinant Human HNRNPU Protein
Recombinant Human HNRNPU Protein is a 127 kDa protein composed of 1186 amino acids. It contains several functional domains, including three RNA recognition motifs (RRMs), a glycine-rich domain, and a serine-rich domain. These domains allow the protein to interact with various RNA molecules, including pre-mRNA, mRNA, and non-coding RNAs.

The three RRMs of HNRNPU are responsible for binding to specific RNA sequences, and the glycine-rich domain is involved in protein-protein interactions. The serine-rich domain, on the other hand, contains multiple phosphorylation sites, which play a crucial role in regulating the activity of HNRNPU.

Activity of Recombinant Human HNRNPU Protein
Recombinant Human HNRNPU Protein is involved in several cellular processes, making it a highly versatile protein. One of its main functions is to regulate alternative splicing, a process that allows a single gene to produce multiple protein isoforms. HNRNPU binds to pre-mRNA and controls the selection of alternative exons, thereby regulating the diversity of protein isoforms.

Moreover, HNRNPU also plays a role in transcription regulation by binding to DNA and regulating the activity of RNA polymerase II. It can either activate or repress gene expression, depending on the specific target gene.

Another essential function of HNRNPU is its involvement in the DNA damage response. It has been shown to interact with several proteins involved in DNA repair, such as BRCA1 and RAD51, and plays a crucial role in maintaining genomic stability.

Applications of Recombinant Human HNRNPU Protein
The versatile nature of HNRNPU makes it a valuable tool in various research applications. Recombinant Human HNRNPU Protein can be used in in vitro studies to investigate the role of this protein in RNA processing and transcription regulation. It can also be used to study the effects of HNRNPU mutations on its activity and function.

Furthermore, Recombinant Human HNRNPU Protein has potential therapeutic applications. It has been shown to be dysregulated in several types of cancer, including breast, ovarian, and lung cancer. Therefore, targeting HNRNPU could be a potential strategy for cancer treatment.

Moreover, HNRNPU has been linked to several neurodevelopmental disorders, such as autism spectrum disorders and intellectual disabilities. Recombinant Human HNRNPU Protein can be used to study the role of this protein in these disorders and potentially develop targeted therapies.

Conclusion
Recombinant Human HNRNPU Protein is a multi-functional protein with a crucial role in various cellular processes. Its structure, activity, and applications make it a valuable tool for research and potential therapeutic applications. Further studies on this protein will provide a better understanding of its role in various biological systems and its potential as a therapeutic target.