Recombinant Human MAST2, N-GST

Description of Recombinant Human MAST2, N-GST

Introduction

Recombinant human MAST2 (Microtubule Associated Serine/Threonine Kinase 2) is a protein that plays a crucial role in regulating cell growth, division, and migration. It is a member of the MAST family of serine/threonine kinases and is encoded by the MAST2 gene on chromosome 1. Recombinant human MAST2 is a synthetic version of the protein produced through genetic engineering techniques, making it a valuable tool for research and therapeutic applications.

Structure of Recombinant Human MAST2

Recombinant human MAST2 is a 180 kDa protein consisting of 1584 amino acids. It contains a catalytic domain, a regulatory domain, and multiple protein-protein interaction domains. The catalytic domain is responsible for the kinase activity of MAST2, while the regulatory domain controls its activity through phosphorylation and protein-protein interactions. The protein-protein interaction domains allow MAST2 to interact with other proteins and regulate various cellular processes.

Activity of Recombinant Human MAST2

Recombinant human MAST2 is a serine/threonine kinase that phosphorylates target proteins, thereby regulating their activity. It is known to phosphorylate a variety of proteins involved in cell signaling, cytoskeleton organization, and cell cycle progression. MAST2 is also involved in the regulation of microtubule dynamics, which is essential for cell division and migration. Additionally, MAST2 has been shown to play a role in cell growth and survival by modulating the activity of the mammalian target of rapamycin (mTOR) pathway.

Application of Recombinant Human MAST2

Recombinant human MAST2 has a wide range of applications in both research and therapeutic settings.

Research Applications

Recombinant human MAST2 is commonly used as a tool in cell signaling and protein-protein interaction studies. Its ability to phosphorylate target proteins allows researchers to study the effects of MAST2 on various cellular processes. It is also used to investigate the role of MAST2 in diseases such as cancer, where its dysregulation has been linked to tumor growth and metastasis.

Therapeutic Applications

The dysregulation of MAST2 has been implicated in several diseases, making it a potential therapeutic target. Recombinant human MAST2 can be used to develop drugs that specifically target MAST2 and its associated pathways. It may also have potential as a biomarker for disease diagnosis and prognosis.

Production of Recombinant Human MAST2

Recombinant human MAST2 is produced through genetic engineering techniques, where the MAST2 gene is inserted into a suitable expression system, such as bacteria or mammalian cells. The protein is then purified using various chromatography techniques to obtain a highly pure and active form of MAST2.

Antigen for Antibody Production

Recombinant human MAST2 can also serve as an antigen for the production of specific antibodies. These antibodies can be used for various applications, such as Western blotting, immunofluorescence, and immunoprecipitation, to study the expression and localization of MAST2 in different cell types and tissues.

Future Directions

With the increasing understanding of MAST2’s role in various cellular processes and diseases, there is a growing interest in the development of MAST2 inhibitors for therapeutic use. Further research on the structure and function of MAST2 may lead to the identification of more specific and potent inhibitors, which could have significant clinical implications.

Conclusion

Recombinant human MAST2 is a key player in regulating cell growth, division, and migration. Its structure, activity, and potential therapeutic applications make it a valuable tool for research and a promising target for drug development. With ongoing research, recombinant human MAST2 may hold the key to understanding