Recombinant Human MICAL1 Protein, N-His

Description of Recombinant Human MICAL1 Protein, N-His

Introduction

Recombinant Human MICAL1 Protein, also known as Microtubule-associated monooxygenase MICAL1, is a protein that plays a crucial role in regulating cell structure and activity. This protein is produced through recombinant DNA technology, making it a highly pure and specific product for various scientific applications. In this article, we will discuss the structure, activity, and applications of Recombinant Human MICAL1 Protein.

Structure of Recombinant Human MICAL1 Protein

Recombinant Human MICAL1 Protein is a member of the MICAL family of proteins, which are characterized by a conserved monooxygenase domain. The protein is composed of 1,289 amino acids and has a molecular weight of approximately 140 kDa. It contains a N-terminal monooxygenase domain, a central calponin homology (CH) domain, and a C-terminal LIM domain. The monooxygenase domain is responsible for the enzymatic activity of the protein, while the CH and LIM domains are involved in protein-protein interactions.

The crystal structure of Recombinant Human MICAL1 Protein has been determined, revealing a unique conformation of the monooxygenase domain. This structure allows the protein to interact with its substrate, actin, and regulate its polymerization and depolymerization. The CH and LIM domains also play a role in the protein’s interaction with other proteins, such as microtubules and cytoskeletal components.

Activity of Recombinant Human MICAL1 Protein

Recombinant Human MICAL1 Protein has been shown to have monooxygenase activity, which is essential for its role in regulating actin dynamics. Through this activity, the protein can oxidize specific methionine residues on actin, leading to changes in its polymerization state. This process is crucial for cell migration, adhesion, and other cellular processes that rely on actin dynamics.

In addition to its monooxygenase activity, Recombinant Human MICAL1 Protein has also been found to have redox activity, which is the ability to transfer electrons between molecules. This activity is important for the protein’s role in regulating oxidative stress and maintaining cellular homeostasis. It has also been linked to the protein’s involvement in neurodegenerative diseases, such as Alzheimer’s and Parkinson’s.

Applications of Recombinant Human MICAL1 Protein

Recombinant Human MICAL1 Protein has a wide range of applications in the scientific field. Its ability to regulate actin dynamics and oxidative stress makes it a valuable tool for studying cellular processes and diseases related to these pathways. Here are some specific applications of this protein:

1. Cell Biology

Recombinant Human MICAL1 Protein is commonly used in cell biology research to study the role of actin in various cellular processes. Its ability to regulate actin polymerization and depolymerization allows researchers to manipulate this process and observe its effects on cell migration, adhesion, and other cellular functions. The protein can also be used to study the role of actin in diseases, such as cancer and cardiovascular diseases.

2. Neuroscience

The redox activity of Recombinant Human MICAL1 Protein has been linked to its involvement in neurodegenerative diseases. As such, this protein is often used in neuroscience research to study the mechanisms of these diseases and potential therapeutic strategies. It has also been found to play a role in neuronal development and plasticity, making it a valuable tool for studying the nervous system.

3. Drug Development

Due to its involvement in various diseases, Recombinant Human MICAL1 Protein has potential as a target for drug development. Researchers are currently exploring the use of this protein as a therapeutic target for neurodegenerative diseases and cancer. The protein can also be used