Recombinant Human NEFH Protein, N-GST & C-His

Description of Recombinant Human NEFH Protein, N-GST & C-His

Introduction

Recombinant Human NEFH Protein, also known as Neurofilament heavy polypeptide, is a protein that plays a crucial role in the structure and function of neurons in the central nervous system. This protein is encoded by the NEFH gene and is a member of the intermediate filament family. Recombinant Human NEFH Protein is produced through genetic engineering techniques, making it a highly purified and biologically active protein with various applications in the field of neuroscience.

Structure of Recombinant Human NEFH Protein

Recombinant Human NEFH Protein is a large protein consisting of 1022 amino acids with a molecular weight of approximately 110 kDa. It is composed of three distinct domains: a head, a rod, and a tail. The head domain contains a high number of negatively charged amino acids, while the rod domain contains a high number of positively charged amino acids. The tail domain, on the other hand, contains a high number of phosphorylation sites, which are important for regulating the assembly and disassembly of the protein.

Activity of Recombinant Human NEFH Protein

The main function of Recombinant Human NEFH Protein is to provide structural support and stability to neurons in the central nervous system. It forms a network of intermediate filaments that extends throughout the length of the axon, providing structural integrity and aiding in the transport of cellular components. It also plays a role in regulating the diameter of the axon, which is important for proper nerve conduction.

In addition to its structural role, Recombinant Human NEFH Protein has been shown to have a neuroprotective function. It has been found to protect neurons from damage caused by oxidative stress and other neurotoxic insults. This is due to its ability to bind to and sequester harmful molecules, preventing them from causing damage to the neuron.

Application of Recombinant Human NEFH Protein

Recombinant Human NEFH Protein has a wide range of applications in the field of neuroscience. One of its main uses is in neurodegenerative disease research. Studies have shown that mutations in the NEFH gene are associated with certain neurodegenerative diseases, such as amyotrophic lateral sclerosis (ALS) and Charcot-Marie-Tooth disease. Recombinant Human NEFH Protein can be used to study the effects of these mutations and to develop potential treatments for these diseases.

Another important application of Recombinant Human NEFH Protein is in nerve injury and regeneration research. Due to its role in providing structural support and aiding in nerve conduction, this protein is crucial for nerve regeneration after injury. Recombinant Human NEFH Protein can be used to study the mechanisms of nerve regeneration and to develop therapies for nerve injuries.

Furthermore, Recombinant Human NEFH Protein has potential applications in the development of biomarkers for neurological disorders. Changes in the levels of this protein have been observed in patients with various neurological diseases, making it a potential biomarker for diagnosis and monitoring of disease progression.

Conclusion

In summary, Recombinant Human NEFH Protein is a vital protein in the central nervous system, playing a crucial role in neuronal structure, function, and protection. Its production through genetic engineering techniques has made it a valuable tool in neuroscience research, with applications ranging from neurodegenerative disease research to nerve injury and regeneration studies. Further research on this protein may lead to a better understanding of neurological disorders and the development of new treatments.