Recombinant Human NUMBL, N-His

Description of Recombinant Human NUMBL, N-His

Introduction

Recombinant Human NUMBL is a protein that plays a crucial role in regulating cell signaling and development. It is a recombinant protein, meaning it is produced through genetic engineering techniques, and has been extensively studied for its structure, activity, and potential applications in various fields of research.

Structure of Recombinant Human NUMBL

Recombinant Human NUMBL is a 65 kDa protein consisting of 591 amino acids. It belongs to the NUMB family of proteins and shares a high degree of sequence homology with its close homolog, NUMB. The protein is composed of several domains, including a phosphotyrosine binding (PTB) domain, a proline-rich region, and a C-terminal domain. These domains play a critical role in the protein’s function and interactions with other molecules.

Activity of Recombinant Human NUMBL

The main function of Recombinant Human NUMBL is to act as a negative regulator of Notch signaling. Notch signaling is a highly conserved pathway that regulates cell fate determination and differentiation. NUMBL binds to the intracellular domain of Notch receptors and prevents their interaction with downstream signaling molecules, thereby inhibiting Notch signaling. This activity is essential for proper cell differentiation and development.

In addition to its role in Notch signaling, Recombinant Human NUMBL has also been shown to interact with other signaling pathways, such as Wnt and Hedgehog, and regulate their activity. It can also act as a scaffold protein, bringing together different molecules involved in signaling pathways and facilitating their interactions.

Applications of Recombinant Human NUMBL

Recombinant Human NUMBL has been extensively studied for its potential applications in various fields of research. Some of the key applications of this protein include:

1. Cancer Research

Aberrant Notch signaling has been linked to the development and progression of various types of cancer. As Recombinant Human NUMBL acts as a negative regulator of Notch signaling, it has been studied as a potential therapeutic target for cancer treatment. Additionally, studies have shown that NUMBL expression is reduced in certain types of cancer, making it a potential biomarker for cancer diagnosis and prognosis.

2. Neurodegenerative Diseases

Notch signaling has been implicated in the pathogenesis of neurodegenerative diseases such as Alzheimer’s and Parkinson’s. Recombinant Human NUMBL, with its ability to regulate Notch signaling, has been studied as a potential therapeutic target for these diseases. It has also been shown to play a role in the development and maintenance of neural stem cells, making it a potential candidate for regenerative medicine in neurological disorders.

3. Stem Cell Research

As mentioned earlier, Recombinant Human NUMBL plays a crucial role in the development and maintenance of stem cells. It has been studied as a potential tool for controlling the fate of stem cells and promoting their differentiation into specific cell types. This has implications in tissue engineering and regenerative medicine, where specific cell types are needed for therapeutic purposes.

4. Developmental Biology

The role of Recombinant Human NUMBL in regulating cell signaling and development makes it a valuable tool for studying developmental processes. Its interaction with various signaling pathways and its ability to act as a scaffold protein make it an essential component in understanding the intricate mechanisms of development.

5. Antibody Production

Recombinant Human NUMBL has also been used in the production of antibodies. It can be used as an antigen to produce specific antibodies that can be used in various research and diagnostic applications.

Conclusion

Recombinant Human NUMBL is a versatile protein that plays a crucial role in regulating cell signaling and development. Its structure, activity, and potential applications have been extensively studied,