Recombinant Human PLS3, N-His

Description of Recombinant Human PLS3, N-His

Introduction to Recombinant Human PLS3, N-His

Recombinant Human PLS3, N-His is a protein that has been genetically engineered for use in scientific research and drug development. It is a variant of the human Plastin 3 (PLS3) protein, which plays a crucial role in cell structure and movement. The recombinant version of this protein has been modified to include a histidine (His) tag, which allows for easier purification and identification in laboratory settings. In this article, we will explore the structure, activity, and potential applications of Recombinant Human PLS3, N-His.

Structure of Recombinant Human PLS3, N-His

The primary structure of Recombinant Human PLS3, N-His is similar to that of the native human PLS3 protein, with the addition of a His tag at the N-terminus. This tag consists of a string of six histidine amino acids, which can bind to nickel or cobalt ions, allowing for purification of the protein using affinity chromatography. The secondary and tertiary structures of Recombinant Human PLS3, N-His are also similar to those of the native protein, with a high degree of alpha-helical content. This structure is important for the protein’s function in cell movement and shape.

Activity of this protein

The primary function of PLS3 is to regulate actin cytoskeleton dynamics, which is essential for cell movement, shape, and division. This is achieved through the binding of PLS3 to actin filaments, causing them to bundle and form structures necessary for cell motility. Recombinant Human PLS3, N-His retains this activity, making it a valuable tool for studying the role of PLS3 in various cellular processes.

In addition to its role in cell movement, PLS3 has also been implicated in diseases such as osteoporosis and cancer. Studies have shown that PLS3 may play a role in bone formation and maintenance, and its expression is altered in certain types of cancer. Recombinant Human PLS3, N-His can be used to investigate the mechanisms underlying these diseases and potentially develop new treatments.

Applications of Recombinant Human PLS3, N-His

One of the main applications of Recombinant Human PLS3, N-His is in drug development. PLS3 has been identified as a potential drug target for diseases such as osteoporosis and cancer, and the recombinant version of this protein can be used to screen for compounds that can modulate its activity. By studying the interaction between PLS3 and these compounds, researchers can identify potential drug candidates for further development.

Recombinant Human PLS3, N-His is also useful in studying the role of PLS3 in various cellular processes. By overexpressing or silencing PLS3 in cells, researchers can observe the effects on cell movement, shape, and division. This can provide valuable insights into the function of PLS3 and its potential role in disease.

Furthermore, Recombinant Human PLS3, N-His can be used in structural studies to determine the three-dimensional structure of PLS3 and its interactions with other proteins or compounds. This information can aid in the design of more effective drugs targeting PLS3 or in the development of new therapeutic strategies.

Conclusion

In summary, Recombinant Human PLS3, N-His is a genetically engineered protein with a histidine tag that retains the activity of the native PLS3 protein. It is a valuable tool for studying the role of PLS3 in cell movement, shape, and disease, as well as for drug development and structural studies. As our understanding of PLS3 continues to grow, Recombinant Human PLS3, N-His will play an important role in advancing scientific research and potentially developing new treatments for various diseases.