Recombinant Human PRRT1 Protein, N-GST & C-His

Description of Recombinant Human PRRT1 Protein, N-GST & C-His

Introduction

Recombinant Human PRRT1 Protein is a protein that is produced through genetic engineering techniques, also known as recombinant protein. It is a member of the peptidase S9B family and is encoded by the PRRT1 gene. This protein plays a crucial role in the regulation of cellular processes and has various applications in the field of biotechnology and medicine. In this article, we will discuss the structure, activity and applications of Recombinant Human PRRT1 Protein.

Structure of Recombinant Human PRRT1 Protein

The Recombinant Human PRRT1 Protein is composed of 280 amino acids and has a molecular weight of approximately 31 kDa. It is a transmembrane protein, with a single transmembrane domain at the C-terminus. The protein has a highly conserved catalytic domain, which is responsible for its peptidase activity. The three-dimensional structure of Recombinant Human PRRT1 Protein has been determined through X-ray crystallography, revealing a globular shape with two distinct domains – the N-terminal and the C-terminal domain.

Activity of Recombinant Human PRRT1 Protein

Recombinant Human PRRT1 Protein is a serine protease that is primarily found in the endoplasmic reticulum and Golgi apparatus. It has been shown to have a broad substrate specificity, with a preference for basic amino acid residues. The protein is involved in the processing and maturation of various proteins, including hormones, growth factors, and enzymes. It also plays a role in the regulation of cell growth, differentiation, and apoptosis.

The peptidase activity of Recombinant Human PRRT1 Protein is dependent on the presence of calcium ions, which act as cofactors for its enzymatic function. The protein has been shown to cleave a variety of substrates, including neuropeptides, hormones, and viral proteins. It has also been found to have a role in the degradation of misfolded or damaged proteins, thus contributing to the quality control of cellular proteins.

Applications of Recombinant Human PRRT1 Protein

Recombinant Human PRRT1 Protein has various applications in the field of biotechnology and medicine. Its peptidase activity makes it a valuable tool for the production of recombinant proteins. The protein can be used to cleave fusion tags from recombinant proteins, allowing for the purification of the target protein. It can also be used in the production of peptides and small proteins through enzymatic digestion.

In the field of medicine, Recombinant Human PRRT1 Protein has been studied for its potential therapeutic applications. It has been shown to have anti-inflammatory properties and has been proposed as a potential treatment for inflammatory diseases such as rheumatoid arthritis and inflammatory bowel disease. The protein has also been found to have a role in the regulation of blood pressure, making it a potential target for the treatment of hypertension.

Moreover, Recombinant Human PRRT1 Protein has been implicated in various pathological conditions, including cancer. It has been found to be upregulated in certain types of cancer, and its overexpression has been associated with tumor growth and metastasis. Therefore, the protein has been studied as a potential target for cancer therapy, with the aim of inhibiting its activity to slow down tumor progression.

Conclusion

In conclusion, Recombinant Human PRRT1 Protein is a versatile protein with a wide range of applications in biotechnology and medicine. Its structure, activity, and various functions make it a valuable tool for the production of recombinant proteins and peptides. Moreover, its potential therapeutic applications and role in disease make it a promising target for future research and development.