Recombinant Human PSMA7 Protein, N-His

Description of Recombinant Human PSMA7 Protein, N-His

Introduction

Recombinant Human PSMA7 Protein is a type of protein that is produced using recombinant DNA technology. It is a highly purified and biologically active form of the human PSMA7 protein, also known as the proteasome subunit alpha type-7. This protein plays a crucial role in the process of protein degradation, which is essential for maintaining cellular homeostasis. In this article, we will discuss the structure, activity, and applications of Recombinant Human PSMA7 Protein.

Structure of Recombinant Human PSMA7 Protein

The PSMA7 gene, located on chromosome 20, encodes the PSMA7 protein. The recombinant form of this protein is produced by inserting the PSMA7 gene into a suitable expression vector, which is then introduced into a host cell. The host cell, usually a bacterial or yeast cell, then produces the protein using its own cellular machinery. The resulting protein is identical to the native human PSMA7 protein in terms of amino acid sequence and structure.

Recombinant Human PSMA7 Protein is a 28 kDa protein consisting of 246 amino acids. It has a globular structure with two distinct domains, the N-terminal domain and the C-terminal domain. The N-terminal domain contains the active site of the protein, which is responsible for its enzymatic activity. The C-terminal domain is involved in protein-protein interactions and is important for the stability and function of the protein.

Activity of Recombinant Human PSMA7 Protein

Recombinant Human PSMA7 Protein is a catalytic subunit of the 20S proteasome, a large protein complex involved in the degradation of intracellular proteins. It is responsible for the chymotrypsin-like activity of the proteasome, which is the ability to cleave peptide bonds after hydrophobic amino acids. This activity is essential for the removal of misfolded or damaged proteins, as well as for the regulation of cellular processes such as cell cycle progression and gene expression.

The activity of Recombinant Human PSMA7 Protein can be measured using various techniques, such as enzymatic assays and western blotting. It has been shown to have a high specific activity and is stable under a wide range of conditions, making it a valuable tool for studying proteasome function.

Applications of Recombinant Human PSMA7 Protein

Recombinant Human PSMA7 Protein has a wide range of applications in both research and industry. Its enzymatic activity and specificity make it a useful tool for studying the function of the proteasome and its role in various cellular processes. It is also used in drug discovery and development, as the proteasome is a target for many diseases, including cancer.

One of the major applications of Recombinant Human PSMA7 Protein is in the production of monoclonal antibodies. Monoclonal antibodies are widely used in research, diagnostics, and therapeutics, and the proteasome is a common target for antibody development. Recombinant Human PSMA7 Protein is often used as an antigen to produce monoclonal antibodies that specifically bind to it, which can then be used for various applications.

In addition, Recombinant Human PSMA7 Protein is also used in the production of protein-based drugs. For example, it has been used as a fusion partner for the production of therapeutic proteins, such as cytokines and growth factors. The fusion protein is then purified using affinity chromatography, with the PSMA7 domain acting as the purification tag. This allows for the efficient and cost-effective production of high-quality therapeutic proteins.

Conclusion

Recombinant Human PSMA7 Protein is a highly purified and biologically active form of the human PSMA7 protein. Its structure, activity, and applications make it a valuable tool for studying proteasome function and for the production of protein-based drugs and monoclonal antibodies. With its diverse applications and potential for further research, Recombinant Human PSMA7 Protein plays a crucial role in advancing