Recombinant Human PTBP3 Protein, N-His

Description of Recombinant Human PTBP3 Protein, N-His

Introduction

Recombinant Human PTBP3 Protein, also known as Polypyrimidine tract-binding protein 3, is a protein that plays a crucial role in regulating gene expression. This protein is encoded by the PTBP3 gene and is found in humans. Recombinant protein refers to a protein that is produced through genetic engineering techniques, rather than being naturally synthesized by an organism. In this article, we will discuss the structure, activity and applications of Recombinant Human PTBP3 Protein.

Structure of Recombinant Human PTBP3 Protein

Recombinant Human PTBP3 Protein is a 58 kDa protein consisting of 534 amino acids. It contains four RNA recognition motifs (RRMs) that are essential for its function. These motifs are responsible for binding to specific RNA sequences and regulating gene expression. The protein also has a nuclear localization signal, indicating that it is localized in the nucleus of the cell.

Activity of Recombinant Human PTBP3 Protein

Recombinant Human PTBP3 Protein is primarily involved in the regulation of alternative splicing, a process that allows a single gene to code for multiple proteins. This protein binds to specific RNA sequences in the pre-mRNA and influences the selection of splice sites, resulting in different protein isoforms. It can also regulate the translation of mRNA into protein by binding to the 3′ untranslated region (UTR) of the mRNA and affecting its stability and translation efficiency.

Moreover, Recombinant Human PTBP3 Protein has been shown to interact with other proteins involved in RNA processing, such as hnRNP proteins and splicing factors, indicating its role in coordinating various steps of gene expression. It has also been reported to play a role in cell proliferation and differentiation, suggesting its involvement in cellular processes beyond gene regulation.

Application of Recombinant Human PTBP3 Protein

Recombinant Human PTBP3 Protein has various applications in both research and medical fields. One of its main applications is in studying alternative splicing and its role in gene expression. By manipulating the expression of PTBP3, researchers can investigate the effects of this protein on alternative splicing and its impact on protein function.

In addition, Recombinant Human PTBP3 Protein has been identified as a potential therapeutic target for diseases such as cancer. Studies have shown that this protein is overexpressed in various types of cancer, and its inhibition can lead to decreased cell proliferation and increased cell death. Therefore, developing drugs that target PTBP3 could potentially be a promising strategy for cancer treatment.

Furthermore, Recombinant Human PTBP3 Protein has been used in diagnostic assays as an antigen for detecting autoantibodies in autoimmune diseases. Autoantibodies against PTBP3 have been found in patients with rheumatoid arthritis, systemic lupus erythematosus, and other autoimmune disorders. Therefore, this protein can serve as a biomarker for these diseases, aiding in their diagnosis and monitoring.

Conclusion

Recombinant Human PTBP3 Protein is a crucial protein involved in regulating gene expression. Its structure, consisting of four RNA recognition motifs, allows it to interact with RNA and other proteins involved in RNA processing. Its activity in alternative splicing and translation makes it a key player in controlling protein diversity and function. The applications of this protein in research and medicine make it a valuable tool for studying gene expression and developing potential therapies for diseases.