Recombinant Human SPIN1, N-His

Description of Recombinant Human SPIN1, N-His

Introduction

Recombinant Human SPIN1 is a protein that has been genetically engineered in the laboratory to mimic the natural human SPIN1 protein. It is a crucial component of various cellular processes and has been extensively studied for its role in cancer development and progression. In this article, we will discuss the structure, activity, and applications of Recombinant Human SPIN1.

Structure of Recombinant Human SPIN1

Recombinant Human SPIN1 is a 79 kDa protein that consists of 703 amino acids. It is composed of several functional domains, including an N-terminal domain, a central proline-rich domain, and a C-terminal domain. The N-terminal domain is responsible for the interaction with other proteins, while the central proline-rich domain is involved in the regulation of protein-protein interactions. The C-terminal domain contains a nuclear localization signal, which is essential for the localization of SPIN1 in the nucleus.

The structure of Recombinant Human SPIN1 is similar to that of the natural human SPIN1 protein, making it a reliable tool for studying the function and mechanisms of SPIN1 in various cellular processes.

Activity of Recombinant Human SPIN1

Recombinant Human SPIN1 is a multifunctional protein that plays a crucial role in various cellular processes, including transcriptional regulation, cell cycle progression, and DNA damage response. It has been shown to interact with several proteins involved in these processes, such as p53, E2F1, and BRCA1.

One of the main activities of Recombinant Human SPIN1 is its role in transcriptional regulation. It has been reported to act as a co-activator of transcription by interacting with transcription factors, such as p53 and E2F1, and enhancing their transcriptional activity. Additionally, SPIN1 has been shown to regulate the expression of genes involved in cell cycle progression, DNA repair, and apoptosis.

Moreover, Recombinant Human SPIN1 has been found to play a crucial role in the DNA damage response. It regulates the activation of the ATM/ATR signaling pathway, which is responsible for sensing and repairing DNA damage. SPIN1 also interacts with BRCA1, a protein involved in DNA repair, and is essential for the recruitment of BRCA1 to sites of DNA damage.

Applications of Recombinant Human SPIN1

The unique structure and activity of Recombinant Human SPIN1 make it a valuable tool for various applications in the field of cancer research. Its role in transcriptional regulation and DNA damage response makes it a potential target for cancer therapy. Additionally, the overexpression of SPIN1 has been linked to various types of cancer, including breast, lung, and liver cancer, making it a potential biomarker for cancer diagnosis and prognosis.

Furthermore, Recombinant Human SPIN1 has been used in studies to understand the mechanisms of cancer development and progression. Its interaction with various proteins involved in cancer-related processes has provided insights into the molecular pathways underlying cancer.

Moreover, Recombinant Human SPIN1 has also been used in drug screening assays to identify potential inhibitors of its activity. These inhibitors can be further developed into targeted therapies for cancer treatment.

Conclusion

In conclusion, Recombinant Human SPIN1 is a genetically engineered protein that mimics the natural human SPIN1 protein. It plays a crucial role in various cellular processes, including transcriptional regulation and DNA damage response. Its unique structure and activity make it a valuable tool for cancer research, with potential applications in cancer therapy, diagnosis, and drug screening. Further studies on Recombinant Human SPIN1 will provide a better understanding of its role in cancer and may lead to the development of new and more effective treatments for this disease.