Recombinant Mouse ST6GALNAC2/SIAT7-B Protein, N-His

Description of Recombinant Mouse ST6GALNAC2/SIAT7-B Protein, N-His

Introduction

Recombinant Mouse ST6GALNAC2/SIAT7-B Protein is a synthetic form of a protein found naturally in mice. This protein is encoded by the ST6GALNAC2 gene and is also known as SIAT7-B due to its function as a sialyltransferase enzyme. Recombinant proteins are created through genetic engineering techniques, allowing for the production of large quantities of specific proteins for use in various scientific and medical applications.

Structure of Recombinant Mouse ST6GALNAC2/SIAT7-B Protein

The protein structure of Recombinant Mouse ST6GALNAC2/SIAT7-B Protein is composed of 384 amino acids, with a molecular weight of approximately 44 kDa. It contains a conserved sialyltransferase domain, which is responsible for its enzymatic activity. This domain is surrounded by several other domains, including a transmembrane domain and a cytoplasmic tail. The protein also has several glycosylation sites, which play a crucial role in its function.

Enzymatic Activity

Recombinant Mouse ST6GALNAC2/SIAT7-B Protein functions as a sialyltransferase enzyme, specifically adding sialic acid residues to the terminal positions of glycoproteins and glycolipids. This process is known as sialylation and is essential for various biological processes, including cell signaling, immune response, and protein stability. The enzyme utilizes CMP-sialic acid as a substrate to transfer sialic acid to the glycan chains of target molecules.

Studies have shown that Recombinant Mouse ST6GALNAC2/SIAT7-B Protein has a preference for specific glycan structures, including alpha-2,6-linked sialic acid residues. This specificity allows for the precise modification of glycoproteins and glycolipids, making it a valuable tool in the field of glycobiology.

Applications of Recombinant Mouse ST6GALNAC2/SIAT7-B Protein

Recombinant Mouse ST6GALNAC2/SIAT7-B Protein has various applications in both scientific research and medical fields. Here are some of its most common uses:

1. Glycan Modification Studies

The ability of Recombinant Mouse ST6GALNAC2/SIAT7-B Protein to precisely add sialic acid residues to glycan chains makes it a valuable tool for studying glycan structures. Researchers can use this protein to investigate the role of sialylation in various biological processes, such as cell signaling, cell adhesion, and protein stability.

2. Glycoprotein Production

Recombinant Mouse ST6GALNAC2/SIAT7-B Protein can also be used to produce glycoproteins with specific sialylation patterns. This is especially useful in the production of therapeutic proteins, where sialylation can improve the stability and efficacy of the protein. This protein can also be used to modify existing glycoproteins, enhancing their therapeutic potential.

3. Diagnostic Applications

The sialylation patterns of glycoproteins can serve as biomarkers for various diseases, including cancer and autoimmune disorders. Recombinant Mouse ST6GALNAC2/SIAT7-B Protein can be used to produce sialylated glycoproteins for use in diagnostic tests, allowing for the early detection and monitoring of these diseases.

4. Vaccine Development

Sialylated glycoproteins have been shown to play a crucial role in the immune response to pathogens. Recombinant Mouse ST6GALNAC2/SIAT7-B Protein can be used to produce sialylated antigens for use in vaccines, potentially enhancing their immunogenic