What types of proteins can you produce in mammalian cells?

We can express virtually any protein type in mammalian cells — including enzymes, receptors, viral proteins, growth factors, and complex biologics. If your protein requires a eukaryotic environment to fold and function correctly, mammalian expression is the right choice.

Which mammalian expression systems and cell lines do you use?

We work with three cell lines to best match your project’s needs: XtenCHO™ Race, our proprietary in-house cell line optimized for high yields and complex PTMs; HEK293T for fast, transient expression; and HEK293F for suspension-based, scalable production. Our team will recommend the most suitable system for your protein.

Do you offer customization for expression vectors and tags?

Yes. We adapt expression vectors and affinity tags to your specific requirements. Whether you need a particular purification tag, a custom promoter, or a specific vector backbone, we work with you to design the optimal construct for your project.

Can you express proteins generated by AI?

Yes. We have successfully completed multiple projects involving AI-designed proteins. Our team is equipped to handle the unique challenges these sequences can present, from codon optimization to expression troubleshooting.

Can you produce glycosylated or other post-translationally modified proteins?

Absolutely. Mammalian cells naturally perform complex post-translational modifications, including glycosylation, phosphorylation, and disulfide bond formation. This is one of the key advantages of our mammalian expression platform, and XtenCHO™ Race is specifically engineered to excel with these demanding proteins.

How long does the process take?

A typical mammalian protein expression project is completed in 2 to 3 weeks. Timelines may vary depending on project complexity, required scale, and any additional services such as characterization. Our team will provide a clear timeline at the start of your project.

Do you handle codon optimization or gene synthesis for mammalian cells?

Yes. We offer full gene synthesis and codon optimization services tailored for mammalian expression systems. This ensures maximum expression efficiency from the very first step of your project.

Can you scale up production for larger quantities?

Yes. Our platform is designed for full scalability — from microgram quantities for early-stage research all the way up to 20L bioreactor production. We also offer stable cell line generation for projects requiring consistent, long-term protein supply.

Mammalian Protein Expression Service

Mammalian Protein Expression Built Around Your Project’s Needs

15 business days production

XtenCHO™ Race: our high-performance proprietary cell line

Scale up to 20L or stable cell line generation

Fast, Flexible Mammalian protein Expression service: Results in as Little as 15 Business Days

XtenCHO™ Race : the Gold Standard for Complex Protein Expression

XtenCHO™ Race protein expression system is ProteoGenix’s proprietary mammalian cell line, developed entirely in-house. It was specifically designed to express complex proteins that require accurate post-translational modifications, which many standard cell lines struggle to reproduce faithfully. Beyond quality, XtenCHO™ Race consistently delivers high protein yields, making it a reliable choice for both research and production scale. And because it is fully IP-free, you can use it without any licensing restrictions or additional costs, keeping your budget under control.

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From Gene to Protein: Flexible Scalable Mammalian Expression

Gene synthesis

Gene design
sequence optimization
gene synthesis
subcloning in expression vector for mammalian cells.

Recombinant expression

Plasmid amplification and preparation
Transfection of target cells
Mammalian cells small scale expression
Expression evaluation by SDS-PAGE analysis (and Western Blot if required)
One-step purification

Recombinant protein QC

Qualitative QC by reducing PAGE analysis (Western Blot if required)
Purity percentage evaluation
Quantitative QC by Bradford or UV280

Talk to our PhD Experts

The Complete Toolkit for end-to-end Protein Development

Protein Characterization Services
- 20+ techniques available
- 1 week starting time
- 2,000+ successful projects
Explore our extensive Characterization panel
High Throughput Protein Expression Screening Service
- Up to x50 protein yield improvement
- 1000 tests in 4 weeks
- Improve all your proteins properties
See our Protein Expression Optimization Service
Cell Line Development Services
- Guaranteed yield estimate
- True proof of monoclonality for regulatory submissions
- Go/No-Go checkpoints
Got to our Stable Cell line development service

Discuss your Project with an Expert

Trusted by Scientists Worldwide

I recently chose ProteoGenix to produce a recombinant protein in HEK293 cells. The high productivity and strong activity of the protein obtained, probably due to proper glycosylation and folding, allowed us to generate exciting results in functional studies and reach new milestones in our research project. The quality of the service provided, and the smooth and kind communication I had with the account manager in charge of our project, were paramount to this success. I’m happy to recommend ProteoGenix for recombinant protein productions

Dr Christian BRONNER, PhD principal scientist, CNRS

Browse through 8,500+ recombinant proteins, enzymes, nanodiscs, miscelles and biologics crafted to the highest quality standards

Fast-Delivery: in your lab in 2-3 days

Look through our extensive panel of proteins

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FAQ

We can express virtually any protein type in mammalian cells — including enzymes, receptors, viral proteins, growth factors, and complex biologics. If your protein requires a eukaryotic environment to fold and function correctly, mammalian expression is the right choice.
We work with three cell lines to best match your project’s needs: XtenCHO™ Race, our proprietary in-house cell line optimized for high yields and complex PTMs; HEK293T for fast, transient expression; and HEK293F for suspension-based, scalable production. Our team will recommend the most suitable system for your protein.
Absolutely. Mammalian cells naturally perform complex post-translational modifications, including glycosylation, phosphorylation, and disulfide bond formation. This is one of the key advantages of our mammalian expression platform, and XtenCHO™ Race is specifically engineered to excel with these demanding proteins.
A typical mammalian protein expression project is completed in 2 to 3 weeks. Timelines may vary depending on project complexity, required scale, and any additional services such as characterization. Our team will provide a clear timeline at the start of your project.