Are you seeking a highly specific, application-ready antibody? With ProteoGenix’s dual-platform approach to custom rabbit antibody discovery, you’ll access the exact antibody you need, tailored to any antigen.

Why choose ProteoGenix custom rabbit monoclonal antibody services?

Guaranteed performance

Select our “Application Guaranteed” packages to ensure your antibody works seamlessly in your desired application (ELISA, sandwich ELISA, Western Blot, flow cytometry, immunofluorescence, immunoprecipitation, immunohistochemistry).

Target any antigen

From non-immunogenic to toxic antigens, our platforms can successfully generate the antibody you need.

Industry-leading turnaround

Receive your antibodies in as few as four weeks.

Animal-free options available

Using our proprietary naïve library, we can develop antibodies without rabbit immunization.

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Custom rabbit antibodies for any target and application

At ProteoGenix, we offer two rabbit antibody discovery platforms. Why two? Because we understand that no single approach can optimally address all antibody discovery challenges, especially given the unique complexities of each antigen and application. By leveraging both platforms, we can provide tailored solutions that maximize success for any antigen, from difficult-to-target non-immunogenic molecules to well-characterized targets requiring high specificity.

When to use Phage Display?

For rabbit antibody development using phage display, it’s essential to consider two scenarios:

  • Naïve SFRab™ Library: Ideal for projects needing rapid turnaround or when immunization isn’t feasible (e.g., toxic, non-immunogenic, or novel antigens). With this fully animal-free library, screening is swift and straightforward.
  • Immune Library: When immunization is possible, an immune library becomes invaluable. While immune libraries may have reduced diversity, they provide a highly specific selection of antibodies against a particular antigen. The antibodies generated through this method often demonstrate exceptional affinity and developability, thanks to in vivo affinity maturation.

When to use single B cell screening?

Single B cell screening is the method of choice for generating rabbit antibodies with high affinity and specificity. This technology isolates B cells from an immunized rabbit based on affinity to the target antigen, resulting in natural VH/VL pairing and optimized developability.
At ProteoGenix, we consider this approach ideal for diagnostic antibody development against known targets. That’s why our diagnostic antibody services with single B cell screening come with an “Application Guaranteed” performance warranty.

Which platform best fits your project needs?

The following table summarizes the strengths and applications of each rabbit monoclonal antibody development platform. This overview helps you choose the optimal strategy for your project, though we recommend further consideration of your target antigen characteristics and final application.

Criteria Single B Cell Screening Phage Display – Naïve Library Phage Display – Immune Library
Affinity +++ + +++
Diversity ++ +++ ++
Specificity +++ + +++
Developability +++ + ++
Use of Rabbits Yes No Yes
Turnaround time ++ + (fastest) +++
Budget ++ + (less expensive) +++
Applications For diagnostics and therapeutics - Ideal for diagnostics with “Application Guaranteed” package Suitable for non-immunogenic/toxic targets or short timelines For diagnostics and therapeutics - Optimized for therapeutic use to discover a large number of binders

Which applications do we guarantee?

ELISA
Sandwich ELISA
Western Blot
Flow cytometry
Immuno fluorescence
Immuno precipitation
Immuno histochemistry
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Case Study: Real results from rabbit monoclonal antibody development

With multiple rabbit antibody development platforms, ProteoGenix addresses diverse project needs. For a closer look at our approach to custom rabbit antibody development via phage display or single B cell screening, download our two case studies below. These reports include:

  • Project description
  • Strategy used for antibody generation
  • Complete set of project results
  • Case report

    Rabbit single B cell screening on protein antigen

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  • Case report

    Phage display screening on protein antigen

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Service details

At ProteoGenix, our team of experts and advanced technologies ensure the best possible approach to your custom rabbit antibody project. Every project begins with a consultation with one of our specialists, where you can provide your project specifications and any target-specific details. We’ll propose the best antibody generation strategy and provide a detailed quote. Once approved, simply submit your antigen sequence, and your project begins!

Rabbit antibody development by single B Cell screening

Step Details Timeline Deliverables
Rabbit Immunization Antigen validation by SDS-Page; immunization with our RocketAbs protocol; bleedings, serum titer tests 29-43 days
Single B-cell Sorting & Screening PBMCs and spleen lymphocyte isolation, single B-cell sorting by FACS, B-cell cultivation, screening 2-3 weeks Up to 30 supernatant samples
Recombinant Rabbit Antibody Production Sequence top clones, transient expression, ELISA screening 2 weeks Up to 10 supernatant samples, report, amino acid sequences of top clones

Rabbit Antibody Development by Phage Display

Step Naïve Library Immune Library Timeline Deliverables
Rabbit Immunization x 29-43 days
PBMC Isolation x 1 week
PCR Amplification x 1 week
Library Construction x 1-2 weeks Final report Library kept by ProteoGenix for further screening service or transfer to customer with full intellectual property
Library Screening (4-6 rounds of library panning) x x
Screening & validation by ELISA x x
Phage DNA extraction + antibody sequencing x x 4-5 weeks from library screening to antibody sequencing Final report DNA sequence of the best binder Full ownership of the sequences
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FAQ on our custom rabbit monoclonal antibody services

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