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Western blotting (also known as protein immunoblotting) is a widely used technique for the detection and characterization of proteins. The technique involves the separation of proteins by size through electrophoresis,… Read more
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Guselkumab Biosimilar – Anti-IL23A mAb – Research Grade

QC & Validation Data
Reference

PX-TA1327

Size

100ug, 1MG

Isotype

IgG1, lambda

Brand

ProteoGenix

Product type

Primary Antibodies

Clonality

Monoclonal Antibody

Expression system

Mammalian cells

Applications

Elisa, WB

$357.00 Select options This product has multiple variants. The options may be chosen on the product page

Anetumab Biosimilar – Anti-MSLN mAb – Research Grade

QC & Validation Data
Reference

PX-TA1330

Size

100ug, 1MG

Isotype

IgG1, lambda

Brand

ProteoGenix

Product type

Primary Antibodies

Clonality

Monoclonal Antibody

Expression system

Mammalian cells

Applications

Elisa, WB

$476.00 Select options This product has multiple variants. The options may be chosen on the product page

Enfortumab Biosimilar – Anti-PVRL4 mAb – Research Grade

QC & Validation Data
Reference

PX-TA1333

Size

100ug, 1MG

Isotype

IgG1, kappa

Brand

ProteoGenix

Product type

Primary Antibodies

Clonality

Monoclonal Antibody

Expression system

Mammalian cells

Applications

Elisa, WB

$476.00 Select options This product has multiple variants. The options may be chosen on the product page

Coltuximab Biosimilar – Anti-CD19 mAb – Research Grade

QC & Validation Data
Reference

PX-TA1345

Size

100ug, 1MG

Isotype

IgG1, kappa

Brand

ProteoGenix

Product type

Primary Antibodies

Clonality

Monoclonal Antibody

Expression system

Mammalian cells

Applications

Elisa, WB

$476.00 Select options This product has multiple variants. The options may be chosen on the product page

Ontuxizumab Biosimilar – Anti-CD248 mAb – Research Grade

QC & Validation Data
Reference

PX-TA1346

Size

100ug, 1MG

Isotype

IgG1, kappa

Brand

ProteoGenix

Product type

Primary Antibodies

Clonality

Monoclonal Antibody

Expression system

Mammalian cells

Applications

Elisa, WB

$476.00 Select options This product has multiple variants. The options may be chosen on the product page

Vanucizumab Biosimilar – Anti-ANGPT2, VEGFA, mAb – Research Grade

QC & Validation Data
Reference

PX-TA1355

Size

100ug, 1MG

Isotype

VH-C-kappa-CH2-CH3_V-lambda-CH1_H-GAMMA-1_L-kappa

Brand

ProteoGenix

Product type

Primary Antibodies

Clonality

Monoclonal Antibody

Expression system

Mammalian cells

Applications

Elisa, WB

$476.00 Select options This product has multiple variants. The options may be chosen on the product page

Imalumab Biosimilar – Anti-MIF mAb – Research Grade

QC & Validation Data
Reference

PX-TA1359

Size

100ug, 1MG

Isotype

IgG1, kappa

Brand

ProteoGenix

Product type

Primary Antibodies

Clonality

Monoclonal Antibody

Expression system

Mammalian cells

Applications

Elisa, WB

$476.00 Select options This product has multiple variants. The options may be chosen on the product page

Lenzilumab Biosimilar – Anti-CSF2 mAb – Research Grade

QC & Validation Data
Reference

PX-TA1360

Size

100ug, 1MG

Isotype

IgG1, kappa

Brand

ProteoGenix

Product type

Primary Antibodies

Clonality

Monoclonal Antibody

Expression system

Mammalian cells

Applications

Elisa, WB

$476.00 Select options This product has multiple variants. The options may be chosen on the product page

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    Western Blot

    Western blotting (also known as protein immunoblotting) is a widely used technique for the detection and characterization of proteins. The technique involves the separation of proteins by size through electrophoresis, followed by transfer of the proteins to a membrane, and finally detection of the proteins with antibodies specific to the target protein of interest.

    Protein Separation

    The first step in a Western blot is to separate the proteins of interest by size through electrophoresis. This is done by placing a sample containing the proteins of interest onto a gel and then applying an electric current to the gel. The proteins are then separated according to their size, with smaller proteins migrating faster than larger proteins.

    Membrane Transfer

    Once the proteins have been separated by size, they are transferred from the gel to a membrane. This is usually done by sandwiching the gel between the membrane and a stack of filter papers, and then applying a current to the membrane. This causes the proteins to be transferred from the gel onto the membrane.

    Antibody Detection

    The final step in a Western blot is the detection of the proteins of interest with antibodies specific to the target protein. To do this, the membrane is first incubated with a primary antibody that binds to the target protein. This is followed by the addition of a secondary antibody that is conjugated to a reporter molecule, such as an enzyme or fluorescent dye. This allows the proteins of interest to be visualized.

    How to choose Antibodies for Western Blotting?

    When choosing antibodies for Western blotting, it is important to select antibodies that have high affinity for the target protein of interest. Antibodies with high affinity will bind more strongly to the target protein, resulting in higher sensitivity and specificity in the detection of the protein. ProteoGenix offers a wide range of high-affinity antibodies for Western blotting, making them an ideal choice for this application.

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