Antigen Retriever is used for staining or to upgrade staining of formalin fixed and paraffin embedded tissues.
It has a wide range of application particularly in immunohistochemistry.
Antigen Retriever 2100 is entirely automated.
The heating pressure and the duration of the cycle for routine formalin-fixed and paraffin embedded tissues is controlled by a chip inside the device.
How does it work:
Antigen Retriever is similar to a pressure cooker.
A microchip within the device dictates the heating profile.
The heating profile is verified and validated for each individual unit of the Retriever during the production.
Similarly, the temperature and the pressure to be reached at a certain pace at a certain time are under the control of specific sensors.
Once the system reaches the required temperature, it stays at that temperature for several minutes.
Then, the slides go through a cooling step for 2 hours.
Special thermal walls control the cooling speed of the inner chamber and the slides. It is worth noting that the cooling step is important, among others, to prevent proteins from re-folding.
Applications:
Antigen Retriever is often used for thermal slide processing of formalin-fixed, paraffin embedded tissues prior to immunostaining.
The model is designed to ensure identical processing of all samples.
Antigen Retriever can be used to:
– Obtain equivalent results every time even in experiments that are achieved months apart
– Run antigen unmasking in up to 6 different buffers in one cycle
– Recover the epitope involved for a strong staining while preserving the tissue and cell morphology
– Use either standard Citrate or EDTA buffers, or our buffers – Use a minimal portion of the processing buffer
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