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View Productssize | 100µg |
---|---|
Isotype | IgG1 |
Brand | ProteoGenix |
Product type | COVID-19 products |
Clonality | Monoclonal Antibody |
Product name | Anti-CoV-Nucleoprotein (G7) antibody |
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Species | Human |
Expression system | Mammalian |
Molecular weight | 150kDa |
Purity | 90% |
Buffer | PBS, pH7,5 |
Form | Liquid |
Delivery condition | Blue ice (+4°C) |
Storage condition | 4°C for short term; -20°c or -80°C for long term |
Brand | Proteogenix |
Reference | PTXCOV-A519A |
Note | For research use and in vitro diagnostic only. Not suitable for human use. |
Isotype | IgG1 |
Clonality | Monoclonal Antibody |
Target | SARS-CoV2 Nucleoprotein |
The anti-CoV-Nucleoprotein (G7) antibody was developed using the
nucleoprotein (protein N or nucleocapsid phosphoprotein) of SARS-CoV-2 as the target antigen. The fully human IgG antibody was initially discovered by screening the exclusive COVID-19 human antibody library (LiAb-SFCOVID-19™) via phage display. The antibody library, designed for a maximal abundance of anti-COVID-19 antibodies, contains the antibody repertoire of dozens of COVID-19 survivors comprising over 1010 different clones.
The nucleoprotein of the new strain of coronavirus is considered the ideal target for designing sensitive SARS-CoV-2 detection assays. This protein is one of the four main structural proteins found in the genome of SARS-CoV-2 and it plays a crucial role in RNA packaging and viral release in the post-infection stage. Nucleoprotein is not exposed on the surface of SARS-CoV-2, but it is one of the most abundantly expressed proteins during infection and proven to be highly immunogenic. Due to its exacerbated expression levels, anti-nucleoprotein antibodies are deemed useful for the detection of SARS-CoV-2 viral particles across different types of samples.
The fully human IgG anti-CoV-Nucleoprotein (G7) antibody was tested and thoroughly validated in enzyme-linked immunosorbent assay (ELISA) using recombinantly expressed nucleoprotein as the target. Moreover, the phage display-derived antibody could be easily produced in the transient mammalian system based on CHO cell lines – XtenCHO™, indicating the high stability (low aggregation potential) of the new molecule.
Anti-CoV-Nucleoprotein (G7) antibody, on SDS-PAGE under reducing and non-reducing condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
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