4°C for short term (1 week), -20°C or -80°C for long term (avoid freezing/thawing cycles; addition of 20-40% glycerol improves cryoprotection)
Brand
ProteoGenix
Host species
Escherichia coli (E.coli)
Fragment Type
Partial
NCBI Reference
NP_588151.1
Aliases /Synonyms
Peptide -N-Glycosidase F, PNGase F, PNGaseF
Reference
PX-P3070
Note
For research use only
Description of Elizabethkingia miricola PNGaseF Recombinant Protein
General information on Elizabethkingia miricola PNGaseF Recombinant Protein-15000u
PNGase F is a recombinant glycosidase cloned from Elizabethkingia miricola and overexpressed in E. coli. It cleaves a complete glycan from a glycoprotein and it deaminates the asparagine to aspartic acid, but leaves the oligosaccharide undamaged. PNGase F will not eliminate oligosaccharides containing Alpha-(1, 3)-linked core fucose usually found in plant glycoproteins. A tri-peptide with the oligosaccharide-linked asparagine as the central residue is the minimal substrate for PNGase F.
1: Kuhn P, Tarentino AL, Plummer TH Jr, Van Roey P. Crystal structure of_x000D_
peptide-N4-(N-acetyl-beta-D-glucosaminyl)asparagine amidase F at 2.2-A_x000D_
resolution. Biochemistry. 1994 Oct 4;33(39):11699-706. PubMed PMID: 7918386.
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2: Norris GE, Stillman TJ, Anderson BF, Baker EN. The three-dimensional structure_x000D_
of PNGase F, a glycosylasparaginase from Flavobacterium meningosepticum._x000D_
Structure. 1994 Nov 15;2(11):1049-59. PubMed PMID: 7881905.
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3: Kuhn P, Guan C, Cui T, Tarentino AL, Plummer TH Jr, Van Roey P. Active site_x000D_
and oligosaccharide recognition residues of_x000D_
peptide-N4-(N-acetyl-beta-D-glucosaminyl)asparagine amidase F. J Biol Chem. 1995 _x000D_
Dec 8;270(49):29493-7. PubMed PMID: 7493989.
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4: Lemp D, Haselbeck A, Klebl F. Molecular cloning and heterologous expression of_x000D_
N-glycosidase F from Flavobacterium meningosepticum. J Biol Chem. 1990 Sep_x000D_
15;265(26):15606-10. PubMed PMID: 2203781.
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5: Barsomian GD, Johnson TL, Borowski M, Denman J, Ollington JF, Hirani S,_x000D_
McNeilly DS, Rasmussen JR. Cloning and expression of_x000D_
peptide-N4-(N-acetyl-beta-D-glucosaminyl)asparagine amidase F in Escherichia_x000D_
coli. J Biol Chem. 1990 Apr 25;265(12):6967-72. PubMed PMID: 2182635.
Waltteri Hosia –
Was the protein active?: Yes
Very good product. I would buy again.