Human sCD14 ELISA Kit

Description of Human sCD14 ELISA Kit

Structure of Human sCD14 ELISA Kit

The Human sCD14 ELISA Kit is a highly sensitive and specific enzyme-linked immunosorbent assay (ELISA) designed for the quantitative detection of soluble cluster of differentiation 14 (sCD14) in human serum, plasma, and cell culture supernatant. The kit contains all necessary components including pre-coated microplate, standards, controls, and reagents for the accurate measurement of sCD14 levels. sCD14 is a glycoprotein that is released from the cell surface by proteolytic cleavage of membrane-bound CD14. It is primarily produced by monocytes, macrophages, and dendritic cells, and is involved in the innate immune response to bacterial lipopolysaccharide (LPS). sCD14 acts as a co-receptor for LPS, facilitating its binding to Toll-like receptor 4 (TLR4) and triggering downstream signaling pathways. It also plays a role in the activation of the complement system and the production of pro-inflammatory cytokines.

The Human sCD14 ELISA Kit utilizes a sandwich ELISA format, where sCD14 present in the sample is captured by a monoclonal antibody coated on the microplate. After washing away unbound materials, a biotinylated detection antibody specific for sCD14 is added, followed by the addition of streptavidin-horseradish peroxidase (HRP) conjugate. The HRP enzyme catalyzes the conversion of a colorless substrate to a colored product, which is measured spectrophotometrically at 450 nm. The intensity of the color is directly proportional to the amount of sCD14 present in the sample.

Activity of Human sCD14 ELISA Kit

The Human sCD14 ELISA Kit has a sensitivity of <5 pg/mL, making it a highly sensitive assay for the detection of sCD14. It has a wide dynamic range of 15.6-1000 pg/mL, allowing for the accurate quantification of both low and high levels of sCD14. The intra-assay and inter-assay coefficients of variation (CVs) are <10% and <12%, respectively, ensuring the precision and reproducibility of the results.

The kit has been validated for use with multiple sample types, including serum, plasma, and cell culture supernatant, making it a versatile tool for various research applications. It has also been validated for use on a variety of ELISA plate readers, providing flexibility for laboratories with different equipment.

In addition to its high sensitivity and accuracy, the Human sCD14 ELISA Kit is also user-friendly and can be completed in less than 4 hours. The kit contains all necessary components and detailed instructions, making it easy to use even for researchers with limited experience in ELISA techniques.

Application of Human sCD14 ELISA Kit

The Human sCD14 ELISA Kit has a wide range of applications in both basic research and clinical settings. One of its main applications is the measurement of sCD14 levels in various biological samples, which can provide valuable insights into the role of sCD14 in different disease states. For example, elevated levels of sCD14 have been observed in sepsis, inflammatory bowel disease, and rheumatoid arthritis, making it a potential biomarker for these conditions.

Furthermore, the Human sCD14 ELISA Kit can be used to study the therapeutic potential of targeting sCD14 in various diseases. As sCD14 plays a critical role in the activation of the immune response, it has been identified as a potential therapeutic target for the treatment of inflammatory and infectious diseases. The kit can be used to measure the levels of sCD14 before and after treatment, providing valuable information on the effectiveness of targeting sCD14.

In summary, the Human sCD14 ELISA Kit is a highly sensitive, accurate, and versatile tool for the detection and quantification of sCD14. Its user