Human ST2 ELISA Kit

Description of Human ST2 ELISA Kit

Introduction

The Human ST2 ELISA Kit is a highly sensitive and specific assay designed for the detection and quantification of soluble ST2 (sST2) in human serum, plasma, and cell culture supernatant. This kit is a valuable tool for researchers and clinicians studying the role of ST2 in various diseases and for the development of potential therapeutic targets.

Structure of ST2

ST2, also known as interleukin-1 receptor-like 1 (IL1RL1), is a member of the interleukin-1 receptor family. It is a transmembrane protein consisting of three domains: an extracellular domain, a transmembrane domain, and an intracellular domain. The extracellular domain contains three immunoglobulin-like domains, while the intracellular domain contains a Toll/interleukin-1 receptor (TIR) domain. sST2 is a soluble form of ST2 that lacks the transmembrane and intracellular domains. It is generated by proteolytic cleavage of the membrane-bound form and is released into the circulation.

Activity of ST2

ST2 is best known as a receptor for interleukin-33 (IL-33), a cytokine involved in immune response and inflammation. Upon binding of IL-33 to ST2, it activates the NF-κB and MAPK signaling pathways, leading to the production of pro-inflammatory cytokines. However, sST2 acts as a decoy receptor for IL-33, preventing its interaction with membrane-bound ST2 and inhibiting the downstream signaling pathways. This suggests that sST2 may have anti-inflammatory effects and play a regulatory role in the immune system.

In addition to its role in immune response, ST2 has been implicated in various diseases. Elevated levels of sST2 have been observed in conditions such as heart failure, asthma, and sepsis, indicating its potential as a biomarker for these diseases. It has also been linked to the pathogenesis of inflammatory disorders, including rheumatoid arthritis and inflammatory bowel disease.

Application as a

Therapeutic Target

Given its involvement in immune response and inflammation, ST2 has emerged as a potential therapeutic target for the treatment of various diseases. Monoclonal antibodies targeting ST2 have shown promising results in preclinical studies for the treatment of asthma, heart failure, and sepsis. These antibodies block the binding of IL-33 to ST2, resulting in reduced inflammation and improved symptoms.

In addition, the use of sST2 as a biomarker has also led to the development of potential therapeutic strategies. For example, in heart failure, elevated levels of sST2 have been associated with poorer outcomes. This has prompted the development of a recombinant form of sST2 as a potential treatment for heart failure. By acting as a decoy receptor for IL-33, it may reduce inflammation and improve cardiac function.

Research Use of Human ST2 ELISA Kit

The Human ST2 ELISA Kit is widely used in research to measure sST2 levels in various biological samples. It offers a simple, reliable, and cost-effective method for the quantitative detection of sST2. This kit has been used in numerous studies investigating the role of ST2 in various diseases, including heart failure, asthma, and sepsis.

Furthermore, the Human ST2 ELISA Kit has been used to assess the efficacy of potential therapeutic interventions targeting ST2. For example, in a study on heart failure patients, the kit was used to measure sST2 levels before and after treatment with a recombinant form of sST2. The results showed a significant decrease in sST2 levels after treatment, suggesting its potential as a therapeutic agent for heart failure.

Conclusion

The Human ST2 ELISA Kit is a valuable tool for researchers and clinicians studying the role of ST2 in various diseases and for the development of potential therapeutic targets