Recombinant ASFV B169L Protein, N-GST

Description of Recombinant ASFV B169L Protein, N-GST

Introduction

Recombinant proteins are proteins that are produced through genetic engineering techniques, where the DNA sequence of a desired protein is inserted into a host organism for production. These proteins have become an important tool in the field of biotechnology and have a wide range of applications in various industries. One such recombinant protein is the ASFV B169L protein, which has gained significant attention in the field of veterinary medicine.

Structure of Recombinant ASFV B169L Protein

The ASFV B169L protein is a ~30 kDa protein that is encoded by the African Swine Fever Virus (ASFV) genome. It is a highly conserved protein and is found in all ASFV strains. The protein is composed of 270 amino acids and has a predicted transmembrane domain at the N-terminus. It also contains a hydrophobic region that is believed to be involved in membrane anchoring. The protein has a globular structure with two domains, a large N-terminal domain and a small C-terminal domain.

Activity of Recombinant ASFV B169L Protein

The ASFV B169L protein is a major structural protein of the ASFV particle and is involved in viral entry and assembly. It is also known to play a role in viral replication and pathogenesis. The protein has been shown to interact with host cell proteins and modulate the host immune response. It is also believed to be involved in the induction of apoptosis in infected cells.

The protein has been extensively studied for its role in viral entry. It has been shown to interact with heparan sulfate proteoglycans on the cell surface, which is believed to be the initial step in viral attachment. The protein is also involved in the fusion of the viral envelope with the host cell membrane, which allows the virus to enter the cell and initiate infection.

Application of Recombinant ASFV B169L Protein

The recombinant ASFV B169L protein has a wide range of applications in the field of veterinary medicine. One of its main applications is in the development of diagnostic tests for ASFV. The protein has been used as an antigen in ELISA and immunoblot assays for the detection of ASFV antibodies in infected animals. It has also been used in the development of rapid diagnostic tests, such as lateral flow assays, for the detection of ASFV in field samples.

The protein has also been studied for its potential as a vaccine candidate against ASFV. Recombinant ASFV B169L protein has been shown to induce a strong immune response in animals, making it a promising candidate for vaccine development. It has also been used in the development of subunit vaccines, where the protein is combined with other ASFV antigens to enhance the immune response.

In addition to its diagnostic and vaccine applications, recombinant ASFV B169L protein has also been used in research studies to better understand the pathogenesis of ASFV. Its role in viral entry and modulation of the host immune response makes it an important protein to study in order to develop effective control measures against ASFV.

Conclusion

Recombinant ASFV B169L protein is a highly conserved protein that plays a crucial role in the entry, replication, and pathogenesis of ASFV. Its structural and functional properties make it an important tool in the development of diagnostic tests and vaccines for ASFV. Further research on this protein is needed to fully understand its role in ASFV infection and to develop effective control measures against this devastating disease.