Recombinant ASFV EP84R Protein, N-His

Description of Recombinant ASFV EP84R Protein, N-His

Introduction

Recombinant ASFV EP84R protein is a highly valuable and versatile antigen that has gained significant attention in the field of immunology. This protein is derived from the African swine fever virus (ASFV), a highly contagious and deadly virus that affects domestic pigs and wild boars. The EP84R protein is a key component of the virus and plays a crucial role in the viral life cycle. In this article, we will explore the structure, activity, and applications of recombinant ASFV EP84R protein.

Structure of Recombinant ASFV EP84R Protein

The recombinant ASFV EP84R protein is a 312 amino acid long polypeptide with a molecular weight of 35 kDa. It is composed of three distinct domains: an N-terminal signal peptide, a central transmembrane domain, and a C-terminal extracellular domain. The N-terminal signal peptide is responsible for the translocation of the protein to the endoplasmic reticulum, where it undergoes post-translational modifications. The central transmembrane domain anchors the protein to the viral envelope, while the C-terminal extracellular domain is exposed on the surface of the virus.

Activity of Recombinant ASFV EP84R Protein

The EP84R protein is a highly active antigen that plays a crucial role in the infection and replication of ASFV. It is involved in the attachment and entry of the virus into host cells, as well as the modulation of the host immune response. The C-terminal extracellular domain of the protein contains multiple epitopes that are recognized by the host immune system, triggering the production of neutralizing antibodies. The transmembrane domain of the protein also plays a role in the fusion of the viral envelope with the host cell membrane, allowing the virus to enter the cell.

Applications of Recombinant ASFV EP84R Protein

Recombinant ASFV EP84R protein has a wide range of applications in the field of immunology. Its ability to induce a strong immune response makes it a valuable antigen for the development of diagnostic tests for ASFV infection. The recombinant protein can be used in ELISA and Western blot assays for the detection of ASFV-specific antibodies in infected animals.

In addition, recombinant ASFV EP84R protein has shown promising results as a potential vaccine candidate against ASFV. Several studies have demonstrated that immunization with the protein can induce a protective immune response in pigs, making them resistant to infection with the virus. This has significant implications for the control and prevention of ASFV outbreaks, which can have devastating effects on the swine industry.

Moreover, the EP84R protein has also been studied for its potential as a therapeutic target for ASFV. Due to its essential role in the viral life cycle, targeting this protein could potentially inhibit viral replication and reduce the severity of the infection. This approach has shown promising results in animal studies, and further research is being conducted to explore its potential as a treatment for ASFV.

Conclusion

In conclusion, recombinant ASFV EP84R protein is a highly valuable antigen with a diverse range of applications in immunology. Its unique structure and activity make it a crucial component of the African swine fever virus, and its potential as a vaccine candidate and therapeutic target has significant implications for the control and prevention of ASFV outbreaks. Further research and development of this protein could lead to improved diagnostic tools, vaccines, and treatments for this deadly virus.