Recombinant DENV-1 Envelope protein E, N-His

Description of Recombinant DENV-1 Envelope protein E, N-His

Introduction

Recombinant DENV-1 Envelope protein E, also known as recombinant protein E or DENV-1 E protein, is a key component of the Dengue virus (DENV). It is a highly conserved protein that is essential for the virus to enter and infect host cells. Recombinant DENV-1 E protein is produced through genetic engineering techniques, making it a valuable tool for research and development of vaccines and diagnostic tests for Dengue virus.

Structure of Recombinant DENV-1 Envelope protein E

The DENV-1 E protein is a glycoprotein that is composed of 495 amino acids. It is a type I transmembrane protein, meaning that it spans the viral membrane and has a portion exposed on the outer surface of the virus. The protein is divided into three domains: domain I, II, and III. Domain I and II are located on the outer surface of the virus and are involved in receptor binding, while domain III is buried within the viral membrane and is responsible for membrane fusion during viral entry.

Recombinant DENV-1 E protein is produced by inserting the gene encoding for the protein into a host cell, such as bacteria or yeast. The protein is then expressed and purified, resulting in a highly pure and stable form of the protein that can be used for various applications.

Activity of Recombinant DENV-1 Envelope protein E

The main function of DENV-1 E protein is to mediate the attachment and entry of the virus into host cells. This is achieved through its interaction with specific receptors on the surface of host cells, such as heparan sulfate and DC-SIGN. The protein also plays a role in viral replication and assembly.

Recombinant DENV-1 E protein has been extensively studied for its ability to induce neutralizing antibodies, which are crucial for protection against Dengue virus infection. Studies have shown that the protein can induce a strong immune response and elicit high levels of neutralizing antibodies in animal models and human subjects.

Application of Recombinant DENV-1 Envelope protein E

Recombinant DENV-1 E protein has a wide range of applications in the field of Dengue virus research and development. One of its key applications is in the development of vaccines against Dengue virus. The protein can be used as a vaccine antigen, either alone or in combination with other viral proteins, to induce a protective immune response against the virus. Several clinical trials have been conducted using recombinant DENV-1 E protein as a vaccine candidate, with promising results.

Another important application of recombinant DENV-1 E protein is in the development of diagnostic tests for Dengue virus. The protein can be used as an antigen in serological tests, such as ELISA and lateral flow assays, to detect the presence of antibodies against the virus in patient samples. This is crucial for accurate diagnosis and surveillance of Dengue virus infections.

Furthermore, recombinant DENV-1 E protein is also used in basic research to study the structure, function, and interactions of the protein with host cells and other viral proteins. It is also a valuable tool for the development of antiviral drugs that target the protein and inhibit its activity.

Conclusion

Recombinant DENV-1 Envelope protein E is a crucial component of the Dengue virus and has a variety of important roles in viral entry, replication, and immune response. Its production through genetic engineering techniques has made it a valuable tool for research and development of vaccines and diagnostic tests for Dengue virus. With ongoing research and development, recombinant DENV-1 E protein holds great promise for the prevention and control of Dengue virus infections.