Recombinant Human ADAMTS12 Protein, N-His

Description of Recombinant Human ADAMTS12 Protein, N-His

Introduction

Recombinant proteins have become an essential tool in various fields of research, diagnostics, and therapeutics. They are produced by genetically engineering DNA sequences to express specific proteins in a host organism. One such protein is ADAMTS12, a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) family. In this article, we will discuss the structure, activity, and applications of recombinant human ADAMTS12 protein.

Structure of Recombinant Human ADAMTS12 Protein

The ADAMTS12 gene is located on chromosome 5 in humans and contains 22 exons. The protein is composed of 1,352 amino acids and has a molecular weight of approximately 150 kDa. The recombinant form of ADAMTS12 protein is produced by inserting the coding sequence of the gene into a suitable expression vector and expressing it in a host cell, such as E. coli or mammalian cells.

The structure of ADAMTS12 protein consists of several functional domains, including a pro-domain, metalloproteinase domain, disintegrin domain, and thrombospondin type 1 motifs. The pro-domain is responsible for the correct folding and activation of the protein. The metalloproteinase domain contains the active site responsible for the proteolytic activity of the enzyme. The disintegrin domain is involved in binding to integrins on the cell surface, while the thrombospondin motifs are responsible for interactions with extracellular matrix proteins.

Activity of Recombinant Human ADAMTS12 Protein

ADAMTS12 is a metalloproteinase enzyme that plays a crucial role in extracellular matrix remodeling. It cleaves various extracellular matrix proteins, including aggrecan, versican, and fibronectin, leading to tissue remodeling and cell migration. The proteolytic activity of ADAMTS12 is regulated by its interaction with tissue inhibitors of metalloproteinases (TIMPs) and other extracellular matrix proteins.

Recent studies have also shown that ADAMTS12 is involved in a variety of biological processes, such as wound healing, angiogenesis, and inflammation. It has been implicated in the pathogenesis of various diseases, including cancer, osteoarthritis, and cardiovascular diseases.

Applications of Recombinant Human ADAMTS12 Protein

The availability of recombinant human ADAMTS12 protein has opened up new avenues for research and development in various fields. One of the major applications of this protein is in the study of its role in various diseases. Recombinant ADAMTS12 can be used to study its interaction with other proteins and its role in extracellular matrix remodeling. It can also be used to screen for potential inhibitors that can modulate its activity and potentially treat diseases associated with its dysregulation.

Recombinant ADAMTS12 protein is also used in diagnostic assays for the detection of ADAMTS12 levels in patient samples. Abnormal levels of ADAMTS12 have been associated with various diseases, and its detection can aid in early diagnosis and treatment.

Furthermore, recombinant ADAMTS12 protein has potential therapeutic applications. It can be used as a drug target for the development of novel therapies for diseases such as cancer and osteoarthritis. In addition, it can also be used as a therapeutic agent itself, either by enhancing or inhibiting its activity, depending on the desired outcome.

Conclusion

In summary, recombinant human ADAMTS12 protein is a valuable tool in scientific research, diagnostics, and therapeutics. Its structure and activity have been extensively studied, and its potential applications continue to expand. With ongoing research, the use of recombinant ADAMTS12 protein is