Recombinant Human ATAD2 Protein, N-His

Description of Recombinant Human ATAD2 Protein, N-His

Introduction

Recombinant Human ATAD2 Protein is a highly purified, bioactive protein that is used in various research and diagnostic applications. It is a recombinant form of the human ATAD2 protein, which is a member of the AAA+ (ATPases associated with diverse cellular activities) superfamily. This protein plays a crucial role in chromatin remodeling, transcriptional regulation, and cell proliferation, making it a valuable tool for studying these processes. In this article, we will discuss the structure, activity, and applications of Recombinant Human ATAD2 Protein.

Structure of Recombinant Human ATAD2 Protein

Recombinant Human ATAD2 Protein is produced through recombinant DNA technology, where the gene encoding the human ATAD2 protein is inserted into a suitable expression vector. The protein is then expressed in a host cell, typically E. coli, and purified to obtain a highly pure and active form of the protein. The recombinant protein has a molecular weight of approximately 150 kDa and contains 1352 amino acids.

The structure of Recombinant Human ATAD2 Protein is characterized by two conserved domains, an N-terminal AAA+ ATPase domain and a C-terminal bromodomain. The AAA+ domain is responsible for the ATPase activity of the protein, which is essential for its function in chromatin remodeling and transcriptional regulation. The bromodomain, on the other hand, is responsible for binding to acetylated lysine residues on histones, allowing the protein to interact with chromatin and regulate gene expression.

Activity of Recombinant Human ATAD2 Protein

Recombinant Human ATAD2 Protein is a multifunctional protein that plays a crucial role in various cellular processes. It is primarily involved in chromatin remodeling, which is the process of altering the structure of chromatin to allow access to DNA for transcription and other cellular processes. The ATPase activity of the protein is essential for this function, as it allows the protein to bind to and remodel chromatin.

In addition to chromatin remodeling, Recombinant Human ATAD2 Protein also plays a role in transcriptional regulation. It has been shown to interact with various transcription factors and co-activators, modulating their activity and influencing gene expression. This protein has also been implicated in cell proliferation, as it is overexpressed in various cancers and has been shown to promote cell growth and survival.

Applications of Recombinant Human ATAD2 Protein

Recombinant Human ATAD2 Protein has a wide range of applications in both research and diagnostics. Its role in chromatin remodeling and transcriptional regulation makes it a valuable tool for studying these processes. Researchers can use this protein to study the effects of chromatin remodeling on gene expression, as well as its role in various diseases, such as cancer.

In diagnostics, Recombinant Human ATAD2 Protein can be used as an antigen for antibody production. Antibodies against this protein can be used for immunohistochemistry and other techniques to detect its expression in tissues and cells. This can provide valuable insights into the role of this protein in various diseases and help in the development of targeted therapies.

Furthermore, Recombinant Human ATAD2 Protein can also be used in drug discovery and development. As this protein is overexpressed in many cancers, it can serve as a potential target for anti-cancer drugs. Researchers can use this protein to screen for compounds that can inhibit its activity and potentially halt the growth of cancer cells.

Conclusion

In conclusion, Recombinant Human ATAD2 Protein is a highly purified and bioactive protein that plays a crucial role in chromatin remodeling, transcriptional regulation, and cell proliferation. Its structure, activity, and applications make it a valuable tool for studying these processes and their role in various diseases. With its potential in diagnostics and drug discovery, this protein holds great promise in advancing our understanding and treatment of various diseases.