Recombinant Human BIRC2 Protein, N-GST

Description of Recombinant Human BIRC2 Protein, N-GST

Introduction

Recombinant Human BIRC2 Protein (also known as Baculoviral IAP Repeat Containing 2) is a member of the inhibitor of apoptosis (IAP) protein family. This protein plays a crucial role in regulating cell death and survival by inhibiting the activity of caspases, which are enzymes responsible for initiating cell death. Recombinant Human BIRC2 Protein is produced using recombinant DNA technology, making it a valuable tool for research and therapeutic applications. In this article, we will discuss the structure, activity, and applications of Recombinant Human BIRC2 Protein.

Structure of Recombinant Human BIRC2 Protein

The human BIRC2 gene is located on chromosome 11 and consists of 11 exons. The protein encoded by this gene is composed of 618 amino acids and has a molecular weight of approximately 69 kDa. Recombinant Human BIRC2 Protein is produced by expressing the BIRC2 gene in a suitable host, such as E. coli or insect cells, and purifying the protein using various chromatography techniques.

The structure of Recombinant Human BIRC2 Protein is characterized by three Baculovirus IAP Repeat (BIR) domains, a ubiquitin-associated (UBA) domain, and a RING finger domain. The BIR domains are responsible for binding to and inhibiting the activity of caspases, while the UBA domain is involved in protein-protein interactions. The RING finger domain is responsible for the E3 ubiquitin ligase activity of BIRC2, which is crucial for regulating protein degradation.

Activity of Recombinant Human BIRC2 Protein

Recombinant Human BIRC2 Protein is a potent inhibitor of apoptosis, which is the process of programmed cell death. This protein exerts its anti-apoptotic activity by binding to and inhibiting the activity of caspases, which are enzymes that play a crucial role in initiating cell death. BIRC2 achieves this by binding to the active site of caspases, preventing them from cleaving their target proteins and initiating the apoptotic cascade.

In addition to its role in regulating apoptosis, Recombinant Human BIRC2 Protein also plays a crucial role in other cellular processes, such as cell proliferation and differentiation. BIRC2 has been shown to interact with various signaling pathways, including the NF-κB pathway, which is involved in regulating immune responses and inflammation. By inhibiting apoptosis and regulating signaling pathways, BIRC2 helps maintain cellular homeostasis and promotes cell survival.

Applications of Recombinant Human BIRC2 Protein

Recombinant Human BIRC2 Protein has a wide range of applications in both research and therapeutic settings. In research, this protein is commonly used as a tool to study the role of BIRC2 in regulating apoptosis and other cellular processes. Recombinant Human BIRC2 Protein can be used to inhibit caspase activity in cell culture experiments, allowing researchers to study the effects of BIRC2 on cell survival and signaling pathways.

In therapeutic applications, Recombinant Human BIRC2 Protein has shown promising potential as a treatment for various diseases. As an inhibitor of apoptosis, BIRC2 can be used to protect cells from undergoing programmed cell death, which can be beneficial in conditions such as neurodegenerative diseases and ischemic injuries. BIRC2 has also been shown to have anti-inflammatory properties, making it a potential therapeutic target for conditions such as rheumatoid arthritis and inflammatory bowel disease.

Conclusion

Recombinant Human BIRC2 Protein is a valuable tool for studying the role of BIRC2 in regulating cell death and survival. Its unique structure and activity make it a potent inhibitor of apoptosis and a potential therapeutic target for various diseases. With ongoing research, the potential applications of Recombinant Human BIRC2 Protein in both research and therapeutic settings will continue to expand, making it an important protein in the field of molecular biology.