Recombinant Human Calprotectin (S100A8/S100A9) Protein, N-His-Strep

Description of Recombinant Human Calprotectin (S100A8/S100A9) Protein, N-His-Strep

Introduction

Recombinant Human Calprotectin (S100A8/S100A9) Protein, also known as MRP8/14 or S100A8/A9, is a heterodimeric protein complex composed of two subunits, S100A8 and S100A9. This protein is a member of the S100 family of calcium-binding proteins and is found in the cytoplasm of various cells, including neutrophils, monocytes, and macrophages. Recombinant Human Calprotectin is produced through recombinant DNA technology and has a wide range of applications in both research and clinical settings.

Structure of Recombinant Human Calprotectin

The structure of Recombinant Human Calprotectin is composed of two subunits, S100A8 and S100A9, which are held together by non-covalent interactions. Each subunit has a molecular weight of approximately 10.8 kDa and is made up of 92 amino acids. The S100A8 subunit contains two calcium-binding EF-hand motifs, while the S100A9 subunit contains four EF-hand motifs. These motifs are responsible for the calcium-dependent conformational changes of the protein.

Activity of Recombinant Human Calprotectin

Recombinant Human Calprotectin has multiple activities and functions in the body. One of its main functions is its role in the innate immune response. This protein acts as a damage-associated molecular pattern (DAMP) molecule, which is released by cells in response to tissue damage or inflammation. It acts as a chemoattractant for immune cells such as neutrophils and monocytes, and also helps to activate these cells to fight against pathogens.

In addition, Recombinant Human Calprotectin has been found to have antimicrobial activity against a wide range of bacteria, fungi, and viruses. It does this by binding to the cell membrane of these microorganisms and disrupting their integrity, leading to cell death.

Application of Recombinant Human Calprotectin

Recombinant Human Calprotectin has a variety of applications in both research and clinical settings. In research, it is commonly used as a biomarker for inflammation and infection. Its levels can be measured in various bodily fluids, such as blood, urine, and synovial fluid, to assess the severity of inflammation and infection.

In addition, Recombinant Human Calprotectin is also used as a tool to study the role of innate immunity in various diseases. Its chemoattractant and antimicrobial properties make it a valuable tool in understanding the immune response to different pathogens.

In clinical settings, Recombinant Human Calprotectin is used as a diagnostic marker for various inflammatory diseases, such as rheumatoid arthritis, inflammatory bowel disease, and sepsis. Its levels have been found to be elevated in these conditions, making it a useful tool for diagnosis and monitoring disease progression.

Furthermore, Recombinant Human Calprotectin has potential therapeutic applications. It has been shown to have anti-inflammatory effects by inhibiting the production of pro-inflammatory cytokines and promoting the production of anti-inflammatory cytokines. This makes it a potential treatment for inflammatory diseases.

Conclusion

In summary, Recombinant Human Calprotectin is a heterodimeric protein complex with multiple functions and activities. Its structure, composed of two subunits, allows for calcium-dependent conformational changes that are essential for its activities. This protein has a wide range of applications in both research and clinical settings, including as a biomarker for inflammation and infection, a tool for studying the immune response, and a potential therapeutic agent for inflammatory diseases. Further research on Recombinant Human Calprotectin may lead to new insights and potential treatments for various diseases.