Recombinant Human CATSPERB Protein, N-His

Description of Recombinant Human CATSPERB Protein, N-His

Introduction

Recombinant Human CATSPERB Protein is a type of protein that has been produced through genetic engineering techniques. It is a key component of the CATSPERB protein family, which plays a crucial role in sperm cell function and fertility. In this article, we will discuss the structure, activity and applications of this important protein.

Structure of Recombinant Human CATSPERB Protein

The CATSPERB protein is composed of 642 amino acids and has a molecular weight of approximately 73 kDa. It is a transmembrane protein, meaning that it spans across the cell membrane. The protein has a large extracellular domain, a single transmembrane domain, and a cytoplasmic domain. The extracellular domain contains several glycosylation sites, which are important for protein stability and function. The cytoplasmic domain is responsible for the protein’s activity and interaction with other cellular components.

Activity of Recombinant Human CATSPERB Protein

The main function of CATSPERB protein is to regulate the movement of calcium ions (Ca2+) into sperm cells. This process, known as sperm capacitation, is essential for sperm cells to become fully functional and capable of fertilizing an egg. CATSPERB protein forms a channel in the sperm cell membrane, through which Ca2+ ions can enter the cell. This influx of Ca2+ ions triggers a series of biochemical reactions that ultimately lead to sperm capacitation.

In addition to its role in sperm capacitation, CATSPERB protein is also involved in sperm motility. It has been shown to interact with other proteins in the CATSPER protein family, forming a complex that is responsible for the movement of sperm cells. This movement is crucial for sperm cells to reach and fertilize an egg.

Applications of Recombinant Human CATSPERB Protein

The discovery of CATSPERB protein and its role in sperm function has opened up new possibilities for its application in various fields. One of the main applications of recombinant CATSPERB protein is in the development of male contraceptives. By targeting the function of CATSPERB protein, scientists are working towards creating a non-hormonal male contraceptive that can effectively prevent pregnancy.

Furthermore, CATSPERB protein has also been studied as a potential target for male infertility treatments. Mutations in the gene that codes for CATSPERB protein have been linked to male infertility, and researchers are exploring ways to restore its function in these cases.

Another potential application of recombinant CATSPERB protein is in assisted reproductive technologies (ART). By understanding the role of this protein in sperm function, scientists can develop new techniques to improve the success rates of ART procedures, such as in vitro fertilization (IVF).

Conclusion

In conclusion, recombinant Human CATSPERB Protein is a crucial component of the CATSPER protein family, playing a vital role in sperm function and fertility. Its structure, activity, and potential applications make it a promising target for various research and medical purposes. Further studies on this protein will continue to expand our understanding of its function and potential uses in the field of reproductive biology.