Recombinant Human EHD1 Protein, N-His

Description of Recombinant Human EHD1 Protein, N-His

Introduction to Recombinant Human EHD1 Protein

Recombinant Human EHD1 Protein, also known as Eps15 homology domain-containing protein 1, is a member of the Eps15 homology (EH) domain-containing protein family. This protein plays a crucial role in regulating endocytic trafficking and membrane dynamics in eukaryotic cells. It is involved in the formation and maintenance of endocytic vesicles, which are responsible for the internalization and recycling of cell surface receptors and other molecules.

Structure of Recombinant Human EHD1 Protein

Recombinant Human EHD1 Protein is a 64-kDa protein consisting of 556 amino acids. It contains an N-terminal EH domain, a central coiled-coil domain, and a C-terminal ATPase domain. The EH domain is responsible for protein-protein interactions, while the coiled-coil domain is involved in oligomerization and membrane binding. The ATPase domain contains conserved motifs required for ATP binding and hydrolysis, which are essential for the protein’s activity.

Activity of Recombinant Human EHD1 Protein

Recombinant Human EHD1 Protein is a key regulator of endocytic trafficking. It functions by binding to and regulating the activity of various proteins involved in endocytosis, such as clathrin, AP-2, and dynamin. It also interacts with membrane lipids, including phosphatidylinositol 4,5-bisphosphate (PIP2), to promote membrane curvature and facilitate vesicle formation.

The ATPase activity of Recombinant Human EHD1 Protein is crucial for its function. It hydrolyzes ATP to ADP, which provides the energy needed for membrane remodeling and vesicle scission. This process is essential for the formation of endocytic vesicles and their subsequent trafficking to different cellular compartments.

Application of Recombinant Human EHD1 Protein

Recombinant Human EHD1 Protein has a wide range of applications in both basic research and therapeutic development. Its role in regulating endocytic trafficking makes it a valuable tool for studying cellular processes such as receptor internalization, recycling, and degradation. It can also be used to investigate the mechanisms of diseases related to endocytic dysfunction, such as cancer and neurodegenerative disorders.

In therapeutic development, Recombinant Human EHD1 Protein has shown potential as a target for drug discovery. Its involvement in endocytosis makes it a promising target for diseases caused by aberrant endocytic trafficking, such as viral infections and lysosomal storage disorders. Additionally, the protein’s ATPase activity makes it a potential target for small molecule inhibitors that can modulate its function.

In conclusion, Recombinant Human EHD1 Protein is a key player in regulating endocytic trafficking and membrane dynamics. Its structure, activity, and applications make it a valuable tool for understanding cellular processes and a potential target for therapeutic development. Further research on this protein is essential for uncovering its full potential and its role in various diseases.