Recombinant Human EIF4B Protein, N-His-SUMO

Description of Recombinant Human EIF4B Protein, N-His-SUMO

Recombinant Human EIF4B Protein, also known as eukaryotic translation initiation factor 4B, is a key component of the eukaryotic translation initiation complex. This protein plays a crucial role in the initiation of protein synthesis by facilitating the binding of the ribosome to the mRNA and promoting the recruitment of the initiator tRNA. Recombinant Human EIF4B Protein is a highly conserved protein, with a molecular weight of approximately 110 kDa, and is encoded by the EIF4B gene located on chromosome 12.

Structure of Recombinant Human EIF4B Protein

The primary structure of Recombinant Human EIF4B Protein consists of 620 amino acids, with a predicted isoelectric point of 5.72. It contains several conserved domains, including the RNA recognition motif (RRM) and the eIF4G binding domain (4G-BD). The RRM domain is responsible for binding to the 5′ cap structure of mRNA, while the 4G-BD domain interacts with eIF4G to form the eIF4F complex, which is essential for translation initiation.

The secondary structure of Recombinant Human EIF4B Protein is predominantly composed of alpha-helices, with a small amount of beta-sheet structure. The protein has a modular architecture, with the RRM and 4G-BD domains connected by a flexible linker region. This allows for the protein to undergo conformational changes and interact with other translation initiation factors during the initiation process.

Activity of Recombinant Human EIF4B Protein

Recombinant Human EIF4B Protein plays a critical role in the initiation of protein synthesis. It is involved in the assembly of the eIF4F complex, which is responsible for recruiting the 40S ribosome to the 5′ end of the mRNA. This process is essential for the efficient translation of mRNA into protein.

In addition to its role in translation initiation, Recombinant Human EIF4B Protein has also been shown to play a role in regulating translation elongation. It has been reported that this protein can interact with the elongation factor eEF2, suggesting that it may also play a role in modulating the rate of protein synthesis.

Application of Recombinant Human EIF4B Protein

Recombinant Human EIF4B Protein has a wide range of applications in both basic research and biotechnology. It is commonly used as an antigen in the development of antibodies for studying the structure and function of this protein. These antibodies can also be used for immunoprecipitation and western blotting to detect and quantify the levels of Recombinant Human EIF4B Protein in different cell types and tissues.

Furthermore, Recombinant Human EIF4B Protein has been used in in vitro translation assays to study the mechanisms of translation initiation and elongation. It has also been used in cell-free translation systems to investigate the role of this protein in regulating protein synthesis in various cellular processes.

In the biotechnology industry, Recombinant Human EIF4B Protein is used in the production of therapeutic proteins, such as insulin and growth factors, by facilitating the translation of recombinant mRNA in cell-free systems. It is also used in the development of novel drugs targeting translation initiation, as dysregulation of this process has been implicated in various diseases, including cancer and neurodegenerative disorders.

In conclusion

Recombinant Human EIF4B Protein is a crucial component of the eukaryotic translation initiation complex, playing a central role in the initiation of protein synthesis. Its highly conserved structure and diverse functions make it an important target for research and biotechnological applications. With its wide range of functions and applications, Recombinant Human EIF4B Protein continues to be an important protein in the study of translation and its role in cellular processes and disease.</